Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
FRCP2; Irisin; Fibronectin type III repeat-containing protein 2
- Product No.APN576Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.6, containing 5% trehalose.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point5.0
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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ACTIVITY TEST of the Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. It was reported that FNDC5 significantly decreased cell number, migration and viability through apoptosis in malignant MDA-MB-231 cells. Thus MDA-MB-231 cells were seeded overnight at a density of 5,000 cells/well, and treated with or without various concentrations of FNDC5 for 48h, then MDA-MB-231 cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then measure the absorbance at 450nm using a microplate reader after incubating the plate for 1-4 hours at 37oC.<br/>Cell apoptosis of MDA-MB-231 cells after incubation with FNDC5 for 48h observed by inverted microscope was shown in Figure 1.<br/>(A) MDA-MB-231 cells cultured in DMEM, stimulated with 4nM FNDC5 for 48h; <br/>(B) Unstimulated MDA-MB-231 cells cultured in DMEM for 48h.<br/>Figure. Cell apoptosis of MDA-MB-231 cells after stimulated with FNDC5.
The dose-effect curve of FNDC5 was shown in Figure 2. It was obvious that FNDC5 significantly decreased cell viability of MDA-MB-231 cells. The ED50 for this effect is typically 1.35~12.52ug/mL.<br/>Figure. The dose-effect curve of FNDC5 on MDA-MB-231 cells.
USAGE of the Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Fibronectin Type III Domain Containing Protein 5 (FNDC5)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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