Active Semaphorin 3A (SEMA3A)
SEMAL; Hsema-I; Hsema-III; SEMA1; SEMAD; SEMAIII; SemD; Coll-1; Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
- Product No.APL917Hu02
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Brand OwnerCLOUD-CLONE CORP.(CCC, USA)
- ManufacturerCLOUD-CLONE CORP.(CCC, WUH)
- Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 90%
- Isoelectric Point8.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 312
For negotiated price and more details, please contact local distributors!US$ 780
For negotiated price and more details, please contact local distributors!US$ 1560
For negotiated price and more details, please contact local distributors!US$ 4680
For negotiated price and more details, please contact local distributors!US$ 117000
For negotiated price and more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant SEMA3A, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
The Semaphorin 3A (SEMA3A) which belongs to the semaphorin family can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Semaphorin 3A is secreted protein containing a Sema domain, an immunoglobulin C2-like domain and a basic domain near the carboxyl tail. It can be secreted by neurons and surrounding tissue to guide migrating cells and axons in the developing nervous system. Besides, Neuropilin 1 (NRP1) has been identified as an interactor of SEMA3A, thus a binding ELISA assay was conducted to detect the interaction of recombinant human SEMA3A and recombinant human NRP1. Briefly, SEMA3A were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to NRP1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-SEMA3A pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of SEMA3A and NRP1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Homo sapiens (Human)||Applications|
|APL917Hu02||Active Semaphorin 3A (SEMA3A)||Cell culture; Activity Assays.|
|RPL917Hu01||Recombinant Semaphorin 3A (SEMA3A)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPL917Hu02||Recombinant Semaphorin 3A (SEMA3A)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAL917Hu01||Polyclonal Antibody to Semaphorin 3A (SEMA3A)||WB; IHC; ICC; IP.|
|PAL917Hu02||Polyclonal Antibody to Semaphorin 3A (SEMA3A)||WB; IHC; ICC; IP.|
|LAL917Hu71||Biotin-Linked Polyclonal Antibody to Semaphorin 3A (SEMA3A)||WB; IHC; ICC.|
|MAL917Hu21||Monoclonal Antibody to Semaphorin 3A (SEMA3A)||WB; IHC; ICC; IP.|
|LAL917Hu72||Biotin-Linked Monoclonal Antibody to Semaphorin 3A (SEMA3A)||WB; IHC; ICC.|
|SEL917Hu||ELISA Kit for Semaphorin 3A (SEMA3A)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCL917Hu||CLIA Kit for Semaphorin 3A (SEMA3A)||Chemiluminescent immunoassay for Antigen Detection.|