Active Paraoxonase 1 (PON1)
ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1
- Product No.APA243Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point5.1
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant PON1, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Paraoxonase 1 (PON1)
Paraoxonase 1 (PON1) is responsible for hydrolysing organophosphate pesticides and nerve gasses. PON1 (paraoxonase 1) is also a major anti-atherosclerotic component of high-density lipoprotein (HDL). Besides, Clusterin (CLU) has been identified as an interactor of PON1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PON1 and recombinant human CLU. Briefly, PON1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to CLU-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-PON1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of PON1 and CLU was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Paraoxonase 1 (PON1)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Paraoxonase 1 (PON1)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Paraoxonase 1 (PON1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPA243Hu01||Recombinant Paraoxonase 1 (PON1)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APA243Hu01||Active Paraoxonase 1 (PON1)||Cell culture; Activity Assays.|
|PAA243Hu01||Polyclonal Antibody to Paraoxonase 1 (PON1)||WB; IHC; ICC; IP.|
|LAA243Hu71||Biotin-Linked Polyclonal Antibody to Paraoxonase 1 (PON1)||WB; IHC; ICC.|
|LAA243Hu81||FITC-Linked Polyclonal Antibody to Paraoxonase 1 (PON1)||WB; IHC; ICC; IF.|
|MAA243Hu22||Monoclonal Antibody to Paraoxonase 1 (PON1)||WB; IHC; ICC; IP.|
|SEA243Hu||ELISA Kit for Paraoxonase 1 (PON1)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|SCA243Hu||CLIA Kit for Paraoxonase 1 (PON1)||Chemiluminescent immunoassay for Antigen Detection.|
|KSA243Hu01||ELISA Kit DIY Materials for Paraoxonase 1 (PON1)||Main materials for "Do It (ELISA Kit) Yourself".|