Active C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
- Product No.APA821Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point5.7
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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FOB
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ACTIVITY TEST of the Active C Reactive Protein (CRP)
C-reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q.Besides,CRP have been proved can promote migration of HepG2 cells, 5×104 cells were seeded into 6 well plates. After cell confluent, using a (yellow) pipette tip make a straight scratch, simulating a wound, then washing the wells three times with PBS. Adding 1% serum standard DMEM containing various concentrations of recombinan rat CRP to each well, incubating the plate for 48 hours at 37℃, 5% CO2. Use Image J to measure the area of a scratch, then caculate the cell motility with (0harea -48harea)/0harea ×100%. After affect with 62.5ng/ml CRP for 48h, cell motility is 47%,wiithout affect by CRP, the cell motility is 27%. The results observed by inverted microscope was shown in Figure1.
USAGE of the Active C Reactive Protein (CRP)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active C Reactive Protein (CRP)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active C Reactive Protein (CRP)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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