Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein

  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Packages (Simulation)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P; Samples: Mouse Kidney Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Mouse Ovary Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Figure. DAB staining on IHC-P;
    Samples: Mouse Spleen Tissue.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Samples: Mouse Uterus Tissue;
    Primary Ab: 10µg/ml Rabbit Anti-Mouse HSPD1 Antibody
    Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Sample: Mouse Uterus Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) DAB staining on IHC-P;
    Sample: Mouse Kidney Tissue
    Primary Ab: 10µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Control: Used PBS instead of primary antibody
    Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Figure: FITC staining on IHC-P;
    Sample: Hela cells.
  • PAA822Mu02.jpg Figure. Western Blot; Sample: Recombinant HSPD1, Mouse.
  • Polyclonal Antibody to Heat Shock Protein 60 (Hsp60) Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: Mouse Lung lysate; Lane3: Hela cell lysate;
    Primary Ab: 3µg/ml Rabbit Anti-Mouse Hsp60 Antibody
    Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

The antibody is a rabbit polyclonal antibody raised against Hsp60. It has been selected for its ability to recognize Hsp60 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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