Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
EMMPRIN; BSG; 5F7; M6; OK; TCSF; Basigin(Ok Blood Group); Collagenase stimulatory factor; OK blood group antigen; Tumor cell-derived collagenase stimulatory factor
- Product No.PAB540Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB540Mu01-Recombinant Cluster Of Differentiation 147 (CD147)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human), Rattus norvegicus (Rat)
- ApplicationsWB; IHC; ICC; IP; FCM
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Testis Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Heart Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Uterus Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Intestine Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant EMMPRIN, Mouse.
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Western Blot; Sample: Lane1: Mouse Liver lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse CD147 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Heart lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse EMMPRIN Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Thymocytes cell were stained with 20µg/ml rabbit Anti-mouse CD147 Polyclonal Antibody (Catalog PAB540Mu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1µg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
The antibody is a rabbit polyclonal antibody raised against CD147. It has been selected for its ability to recognize CD147 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cluster Of Differentiation 147 (CD147)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| APB540Mu61 | Active Cluster Of Differentiation 147 (CD147) | Cell culture; Activity Assays. |
| APB540Mu02 | Active Cluster Of Differentiation 147 (CD147) | Cell culture; Activity Assays. |
| RPB540Mu01 | Recombinant Cluster Of Differentiation 147 (CD147) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB540Mu02 | Recombinant Cluster Of Differentiation 147 (CD147) | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB540Mu01 | Active Cluster Of Differentiation 147 (CD147) | Cell culture; Activity Assays. |
| EPB540Mu61 | Eukaryotic Cluster Of Differentiation 147 (CD147) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB540Mu01 | Polyclonal Antibody to Cluster Of Differentiation 147 (CD147) | WB; IHC; ICC; IP; FCM |