Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
CCS-3; CCS3; EEF-1; EEF1A; EF-Tu; EF1A; FLJ25721; GRAF-1EF; LENG7; PTI1; eEF1A-1; Leukocyte Receptor Cluster(LRC)Member 7; Elongation factor Tu
- Product No.PAF020Hu01
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPF020Hu01-Recombinant Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreMus musculus (Mouse)
- ApplicationsWB; IHC; ICC/IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
-
FOB
US$ 105
For more details, please contact local distributors!US$ 245
For more details, please contact local distributors! US$ 350
For more details, please contact local distributors! US$ 875
For more details, please contact local distributors! US$ 3500
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20µg/ml Rabbit Anti-Human EEF1a1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
-
DAB staining on IHC-P;
Sample: Rat Colon Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 20µg/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human EEF1a1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Human EEF1a1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Human EEF1a1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;
Sample: HepG2 cell
Primary Ab: 20µg/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
-
Figure. Western Blot; Sample: Recombinant EEF1a1, Human.
-
Western Blot; Samples: Lane1: Hela cell lysate; Lane2: Jurkat cell lysate; Lane3: Mouse Heart lysate; Lane4: Mouse Cerebrum lysate;
Primary Ab: 1µg/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 0.2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Mouse Heart lysate; Lane2: Mouse Cerebrum lysate; Lane3: Hela cell lysate; Lane4: Jurkat cell lysate; Lane5: U2OS cell lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: EEF1a1 knockout Jurkat cell lysate;
Predicted MW: 48,50kd
Observed MW: 50kd
Primary Ab: 1µg/ml Rabbit Anti-Human EEF1a1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
-
ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
The antibody is a rabbit polyclonal antibody raised against EEF1a1. It has been selected for its ability to recognize EEF1a1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
| RPF020Hu01 | Recombinant Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF020Hu01 | Polyclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) | WB; IHC; ICC/IF |
| MAF020Hu21 | Monoclonal Antibody to Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) | WB; IHC; ICC; IP. |
| SEF020Hu | ELISA Kit for Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF020Hu | Multiplex Assay Kit for Eukaryotic Translation Elongation Factor 1 Alpha 1 (EEF1a1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |