Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
SNC-A; aSYN; PD1; PARK1; PARK4; NACP; Non-A Beta Component Of Alzheimer's Disease Amyloid Precursor Protein; Non-A4 component of amyloid precursor
- Product No.MEB222Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 33pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 575
For more details, please contact local distributors!US$ 821
For more details, please contact local distributors! US$ 3695
For more details, please contact local distributors! US$ 6979
For more details, please contact local distributors! US$ 57470
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
This assay has high sensitivity and excellent specificity for detection of Mini Samples Synuclein Alpha (SNCa).
No significant cross-reactivity or interference between Mini Samples Synuclein Alpha (SNCa) and analogues was observed.
Recovery of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
Matrices listed below were spiked with certain level of recombinant Mini Samples Synuclein Alpha (SNCa) and the recovery rates were calculated by comparing the measured value to the expected amount of Mini Samples Synuclein Alpha (SNCa) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 82-89 | 86 |
| EDTA plasma(n=5) | 80-101 | 86 |
| heparin plasma(n=5) | 89-101 | 96 |
Precision of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Synuclein Alpha (SNCa) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Synuclein Alpha (SNCa) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Mini Samples Synuclein Alpha (SNCa) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-99% | 92-101% | 85-94% | 84-101% |
| EDTA plasma(n=5) | 83-95% | 88-96% | 78-99% | 89-102% |
| heparin plasma(n=5) | 86-103% | 94-102% | 94-101% | 97-104% |
Stability of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
1. Prepare all reagents, samples and standards;
2. Add 25µL standard or sample to each well. Incubate 1 hour at 37°C;
3. Aspirate and add 25µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 25µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 25µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 20µL Stop Solution. Read at 450nm immediately.
Test principle of the Mini Samples ELISA Kit for Synuclein Alpha (SNCa)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Synuclein Alpha (SNCa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Synuclein Alpha (SNCa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Synuclein Alpha (SNCa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Synuclein Alpha (SNCa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPB222Mu01 | Recombinant Synuclein Alpha (SNCa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB222Mu02 | Recombinant Synuclein Alpha (SNCa) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB222Mu02 | Polyclonal Antibody to Synuclein Alpha (SNCa) | WB; IHC; ICC; IP. |
| PAB222Mu01 | Polyclonal Antibody to Synuclein Alpha (SNCa) | WB; IHC; ICC; IP. |
| SEB222Mu | ELISA Kit for Synuclein Alpha (SNCa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEB222Mu | Mini Samples ELISA Kit for Synuclein Alpha (SNCa) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB222Mu | Multiplex Assay Kit for Synuclein Alpha (SNCa) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSB222Mu01 | ELISA Kit DIY Materials for Synuclein Alpha (SNCa) | Main materials for "Do It (ELISA Kit) Yourself". |