Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

P1NP; N-Propeptide Of Type I Procollagen; Procollagen I Amino Terminal Propeptide

  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)Packages (Simulation)
  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)Packages (Simulation)
  • Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)Results demonstration
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

This assay has high sensitivity and excellent specificity for detection of Instant Procollagen I N-Terminal Propeptide (PINP).
No significant cross-reactivity or interference between Instant Procollagen I N-Terminal Propeptide (PINP) and analogues was observed.

Recovery of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Matrices listed below were spiked with certain level of recombinantInstant Procollagen I N-Terminal Propeptide (PINP) and the recovery rates were calculated by comparing the measured value to the expected amount of Instant Procollagen I N-Terminal Propeptide (PINP) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)78-10283
EDTA plasma(n=5)83-10196
heparin plasma(n=5)82-9688

Precision of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Instant Procollagen I N-Terminal Propeptide (PINP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Instant Procollagen I N-Terminal Propeptide (PINP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)98-105%79-104%79-91%92-99%
EDTA plasma(n=5)96-103%99-105%84-98%88-102%
heparin plasma(n=5)91-105%80-90%80-102%94-101%

Stability of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard5Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample and 50µL Detection Reagent A to each well. Incubate 1 hours at 37°C;
3. Aspirate and wash 3 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450nm immediately.

Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

Test principle of the Instant ELISA Kit for Procollagen I N-Terminal Propeptide (PINP)

The test principle applied in this kit is enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP). Standards or samples and HRP-labeled detection antibody specific to Instant Procollagen I N-Terminal Propeptide (PINP) (Detection Reagent A) are then added to the appropriate microtiter plate wells. Next, TMB substrate solution is added, only those wells that contain Instant Procollagen I N-Terminal Propeptide (PINP), and HRP-labeled detection antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Instant Procollagen I N-Terminal Propeptide (PINP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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