ELISA Kit for Ovalbumin (OVA)

  • ELISA Kit for Ovalbumin (OVA) Packages (Simulation)
  • ELISA Kit for Ovalbumin (OVA) Packages (Simulation)
  • ELISA Kit for Ovalbumin (OVA) Results demonstration
  • CEB459Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Ovalbumin (OVA)

This assay has high sensitivity and excellent specificity for detection of Ovalbumin (OVA).
No significant cross-reactivity or interference between Ovalbumin (OVA) and analogues was observed.

Recovery of the ELISA Kit for Ovalbumin (OVA)

Matrices listed below were spiked with certain level of recombinant Ovalbumin (OVA) and the recovery rates were calculated by comparing the measured value to the expected amount of Ovalbumin (OVA) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 96-104 101
EDTA plasma(n=5) 83-97 89
heparin plasma(n=5) 83-103 92

Precision of the ELISA Kit for Ovalbumin (OVA)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ovalbumin (OVA) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ovalbumin (OVA) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Ovalbumin (OVA)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Ovalbumin (OVA) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-98% 79-101% 89-101% 81-88%
EDTA plasma(n=5) 85-94% 84-92% 99-105% 92-105%
heparin plasma(n=5) 81-105% 89-102% 94-101% 84-95%

Stability of the ELISA Kit for Ovalbumin (OVA)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Ovalbumin (OVA)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Ovalbumin (OVA)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ovalbumin (OVA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ovalbumin (OVA) and unlabeled Ovalbumin (OVA) (Standards or samples) with the pre-coated antibody specific to Ovalbumin (OVA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Ovalbumin (OVA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Ovalbumin (OVA) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
NPB459Ge01 Native Ovalbumin (OVA) Positive Control; Immunogen; SDS-PAGE; WB.
PAB459Ge01 Polyclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
LAB459Ge71 Biotin-Linked Polyclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC.
MAB459Ge21 Monoclonal Antibody to Ovalbumin (OVA) WB
MAB459Ge24 Monoclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
MAB459Ge23 Monoclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
MAB459Ge25 Monoclonal Antibody to Ovalbumin (OVA) WB
MAB459Ge22 Monoclonal Antibody to Ovalbumin (OVA) WB; IHC; ICC; IP.
HEB459Ge High Sensitive ELISA Kit for Ovalbumin (OVA) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB459Ge ELISA Kit for Ovalbumin (OVA) Enzyme-linked immunosorbent assay for Antigen Detection.
CEB459Ge ELISA Kit for Ovalbumin (OVA) Enzyme-linked immunosorbent assay for Antigen Detection.
AEB459Ge ELISA Kit for Anti-Ovalbumin Antibody (Anti-OVA) Enzyme-linked immunosorbent assay for Antibody Detection.
CCB459Ge CLIA Kit for Ovalbumin (OVA) Chemiluminescent immunoassay for Antigen Detection.
LMB459Ge Multiplex Assay Kit for Ovalbumin (OVA) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSB459Ge11 ELISA Kit DIY Materials for Ovalbumin (OVA) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK