ELISA Kit for Aspartate Aminotransferase (AST)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

  • ELISA Kit for Aspartate Aminotransferase (AST) Packages (Simulation)
  • ELISA Kit for Aspartate Aminotransferase (AST) Packages (Simulation)
  • ELISA Kit for Aspartate Aminotransferase (AST) Results demonstration
  • SEB214Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Aspartate Aminotransferase (AST)

This assay has high sensitivity and excellent specificity for detection of Aspartate Aminotransferase (AST).
No significant cross-reactivity or interference between Aspartate Aminotransferase (AST) and analogues was observed.

Recovery of the ELISA Kit for Aspartate Aminotransferase (AST)

Matrices listed below were spiked with certain level of recombinant Aspartate Aminotransferase (AST) and the recovery rates were calculated by comparing the measured value to the expected amount of Aspartate Aminotransferase (AST) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 102
EDTA plasma(n=5) 84-103 101
heparin plasma(n=5) 95-104 101

Precision of the ELISA Kit for Aspartate Aminotransferase (AST)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Aspartate Aminotransferase (AST) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Aspartate Aminotransferase (AST)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Aspartate Aminotransferase (AST) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 89-101% 94-102% 81-89% 87-101%
EDTA plasma(n=5) 80-103% 89-98% 86-94% 79-101%
heparin plasma(n=5) 83-97% 97-105% 93-101% 93-101%

Stability of the ELISA Kit for Aspartate Aminotransferase (AST)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Aspartate Aminotransferase (AST)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Aspartate Aminotransferase (AST)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Aminotransferase (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Aminotransferase (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Aminotransferase (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Aminotransferase (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPB214Hu01 Recombinant Aspartate Aminotransferase (AST) Positive Control; Immunogen; SDS-PAGE; WB.
EPB214Hu51 Eukaryotic Aspartate Aminotransferase (AST) Positive Control; Immunogen; SDS-PAGE; WB.
APB214Hu01 Active Aspartate Aminotransferase (AST) Cell culture; Activity Assays.
APB214Hu51 Active Aspartate Aminotransferase (AST) Cell culture; Activity Assays.
PAB214Hu07 Polyclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IP.
PAB214Hu01 Polyclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IP.
PAB214Hu51 Polyclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IP.
LAB214Hu81 FITC-Linked Polyclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IF.
LAB214Hu71 Biotin-Linked Polyclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC.
MAB214Hu21 Monoclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC
MAB214Hu22 Monoclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IP.
MAB214Hu23 Monoclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC; IP.
FAB214Hu02 Anti-Aspartate Aminotransferase (AST) Monoclonal Antibody Flow cytometry.
LAB214Hu72 Biotin-Linked Monoclonal Antibody to Aspartate Aminotransferase (AST) WB; IHC; ICC.
MEB214Hu Mini Samples ELISA Kit for Aspartate Aminotransferase (AST) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB214Hu ELISA Kit for Aspartate Aminotransferase (AST) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB214Hu Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
PSB214Hu01 Antibody Pair for Aspartate Aminotransferase (AST) ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB214Hu01 ELISA Kit DIY Materials for Aspartate Aminotransferase (AST) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK