Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein; Pyruvate Kinase, Muscle
- Product No.PAA588Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA588Ra01-Recombinant Pyruvate kinase isozymes M2 (PKM2)
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species MoreHomo sapiens (Human)
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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For more details, please contact local distributors!US$ 239
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For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Testis Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Intestine Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue
Primary Ab: 10µg/ml Rabbit Anti-Rat PKM2 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Lung Tissue;
Primary Ab: 10ug/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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WB Image
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Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Hela cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat PKM2 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-rat PKM2 Polyclonal Antibody (Catalog # PAA588Ra01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA588Ra01 | Recombinant Pyruvate kinase isozymes M2 (PKM2) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA588Ra01 | Polyclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) | WB; IHC; ICC; IP. |
| FAA588Ra01 | Anti-Pyruvate kinase isozymes M2 (PKM2) Polyclonal Antibody | Flow cytometry. |
| MAA588Ra21 | Monoclonal Antibody to Pyruvate kinase isozymes M2 (PKM2) | WB; IHC; ICC; IP. |
| SEA588Ra | ELISA Kit for Pyruvate kinase isozymes M2 (PKM2) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA588Ra | Multiplex Assay Kit for Pyruvate kinase isozymes M2 (PKM2) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |