Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

M2PK; PKM; M2-PK; PKM1; CTHBP; OIP3; PK3; PKM; TCB; THBP1; Pyruvate kinase muscle isozyme; Thyroid hormone-binding protein 1; Cytosolic thyroid hormone-binding protein

  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Packages (Simulation)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Packages (Simulation)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Used in DAB staining on fromalin fixed paraffin- embedded kidney tissue
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)DAB staining on IHC-P;
    Samples: Human Skin cancer Tissue
  • PAA588Hu01.jpgFigure. Western Blot; Sample: Recombinant PKM2, Human.
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Western Blot; Sample: Human K562 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Knockout Varification:
    Lane 1: Wild-type MCF7 cell lysate;
    Lane 2: PKM2 knockout MCF7 cell lysate;
    Predicted MW: 56,58kd
    Observed MW: 60kd
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Western Blot; Sample: Human MCF7 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Western Blot; Sample: Human Hela cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)Western Blot; Sample: Human HepG2 cell lysate;
    Primary Ab: 2µg/ml Rabbit Anti-Human PKM2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)K562 human chronic myelogenous leukemia cell line was stained with rabbit Anti-human PKM2 Polyclonal Antibody (Catalog # PAA588Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

The antibody is a rabbit polyclonal antibody raised against PKM2. It has been selected for its ability to recognize PKM2 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
RPA588Hu01Recombinant Pyruvate Kinase, Muscle (PKM2)Positive Control; Immunogen; SDS-PAGE; WB.
RPA588Hu02Recombinant Pyruvate Kinase, Muscle (PKM2)Positive Control; Immunogen; SDS-PAGE; WB.
PAA588Hu02Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)WB; IHC; ICC; IP.
PAA588Hu01Polyclonal Antibody to Pyruvate Kinase, Muscle (PKM2)WB; IHC; ICC; IP.
MAA588Hu22Monoclonal Antibody to Pyruvate Kinase, Muscle (PKM2)WB; IHC; ICC; IP.
SEA588HuELISA Kit for Pyruvate Kinase, Muscle (PKM2)Enzyme-linked immunosorbent assay for Antigen Detection.
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