Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)
HMG1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein
- Product No.PAA399Ra01
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA399Ra01-Recombinant High Mobility Group Protein 1 (HMGB1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreHomo sapiens (Human), Mus musculus (Mouse)
- ApplicationsWB,IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 103
For more details, please contact local distributors!US$ 239
For more details, please contact local distributors! US$ 342
For more details, please contact local distributors! US$ 855
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Rat Stomach Tissue; Primary Ab: 10µg/ml Rabbit Anti-Rat HMG1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Rat Pancreas Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Spleen Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat HMGB1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Colon Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat HMGB1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Uterus Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat HMGB1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat HMGB1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Stomach Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat HMGB1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Spinal cord Tissue
Primary Ab: 20µg/ml Rabbit Anti-Rat HMGB1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant HMG1, Rat.
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Western Blot; Sample: Human Jurkat cell lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: HMG1 knockout Jurkat cell lysate;
Predicted MW: 25kd
Observed MW: 28kd
Primary Ab: 2µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Cerebrum lysate; Lane3: Hela cell lysate; Lane4: HepG2 cell lysate; Lane5: A431 cell lysate
Primary Ab: 0.1µg/ml Rabbit Anti-Rat HMGB1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2µg/ml Rabbit Anti-Rat HMG1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)
The antibody is a rabbit polyclonal antibody raised against HMGB1. It has been selected for its ability to recognize HMGB1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPA399Ra01 | Recombinant High Mobility Group Protein 1 (HMGB1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA399Ra01 | Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB,IHC |
| LAA399Ra81 | FITC-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC; IF. |
| LAA399Ra71 | Biotin-Linked Polyclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC. |
| MAA399Ra21 | Monoclonal Antibody to High Mobility Group Protein 1 (HMGB1) | WB; IHC; ICC; IP. |
| SEA399Ra | ELISA Kit for High Mobility Group Protein 1 (HMGB1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA399Ra | Multiplex Assay Kit for High Mobility Group Protein 1 (HMGB1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
| KSA399Ra01 | ELISA Kit DIY Materials for High Mobility Group Protein 1 (HMGB1) | Main materials for "Do It(ELISA Kit) Yourself" |