Polyclonal Antibody to Complement Component 4a (C4a)
C4a anaphylatoxin
- Product No.PAA389Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPA389Mu02-Recombinant Complement Component 4a (C4a)
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB; IHC
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Liver Tissue; Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Mouse Kidney Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Colon Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Mouse Small intestine Tissue;
Primary Ab: 30µg/ml Rabbit Anti-Mouse C4a Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Liver Tissue
Primary Ab: 10µg/ml Rabbit Anti-Mouse C4a Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Mouse Plasma; Lane2: Rat Serum; Lane3: Rat Plasma Primary Ab: 1μg/ml Rabbit Anti-Mouse C4a Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Mouse Placenta lysate
Primary Ab: 0.2µg/ml Rabbit Anti-Mouse C4a Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) Selected
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Complement Component 4a (C4a)
The antibody is a rabbit polyclonal antibody raised against C4a. It has been selected for its ability to recognize C4a in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Complement Component 4a (C4a)
Western blotting: 0.01-3µg/mL;
Immunohistochemistry: 5-30µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Complement Component 4a (C4a)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Complement Component 4a (C4a)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA389Mu01 | Recombinant Complement Component 4a (C4a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA389Mu02 | Recombinant Complement Component 4a (C4a) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA389Mu02 | Polyclonal Antibody to Complement Component 4a (C4a) | WB; IHC; ICC; IP. |
| PAA389Mu01 | Polyclonal Antibody to Complement Component 4a (C4a) | WB; IHC |
| SEA389Mu | ELISA Kit for Complement Component 4a (C4a) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA389Mu | Multiplex Assay Kit for Complement Component 4a (C4a) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |
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