Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule

  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Packages (Simulation)
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Packages (Simulation)
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Figure. DAB staining on IHC-P; Samples:Human Liver Cancer Tissue.
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Figure. DAB staining on IHC-P; Samples:Human Prostate Tissue.
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Figure. DAB staining on IHC-P; Samples:Human Kidney Tissue.
  • PAA781Hu01.jpgFigure. Western Blot; Sample: Recombinant CD276, Human.
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Figure. Western Blot; Sample: Lane1: Human 293T Cells; Lane2: Human PC-3 Cells; Lane3: Human MCF-7 Cells.
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)Knockout Varification:
    Lane 1: Wild-type 293T cell lysate;
    Lane 2: CD276 knockout 293T cell lysate;
    Predicted MW: 57,53,33kd
    Observed MW: 100kd
    Primary Ab: 3µg/ml Rabbit Anti-Human CD276 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)ChoK1 transfected with human CD276(Stable transfection) was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

The antibody is a rabbit polyclonal antibody raised against CD276. It has been selected for its ability to recognize CD276 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
APA781Hu01Active Cluster Of Differentiation 276 (CD276)Cell culture; Activity Assays.
RPA781Hu01Recombinant Cluster Of Differentiation 276 (CD276)Positive Control; Immunogen; SDS-PAGE; WB.
PAA781Hu01Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)WB; IHC; ICC; IP.
MAA781Hu22Monoclonal Antibody to Cluster Of Differentiation 276 (CD276)WB; IHC; ICC; IP.
SEA781HuELISA Kit for Cluster Of Differentiation 276 (CD276)Enzyme-linked immunosorbent assay for Antigen Detection.
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