Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
CED4; Apoptotic Protease Activating Factor
- Product No.PAB054Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig Type IgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- Immunogen RPB054Mu01-Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration0.5mg/ml
- Organism Species MoreRattus norvegicus (Rat)
- ApplicationsWB; IHC; IF
If the antibody is used in flow cytometry, please check FCM antibodies. - Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Samples: Mouse Cardiac Muscle Tissue; Primary Ab: 20ug/ml Rabbit Anti-Mouse APAF1 Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Mouse Kidney Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Mouse APAF1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Kidney Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse APAF1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Mouse Cardiac Muscle Tissue
Primary Ab: 20µg/ml Rabbit Anti-Mouse APAF1 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2µg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Mouse APAF1 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Sample: Hela cell
Primary Ab: 20µg/ml Rabbit Anti-Mouse APAF1 Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant APAF1, Mouse.
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
The antibody is a rabbit polyclonal antibody raised against APAF1. It has been selected for its ability to recognize APAF1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
Western blotting: 0.01-2µg/mL;
Immunohistochemistry: 5-20µg/mL;
Immunocytochemistry: 5-20µg/mL;
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPB054Mu02 | Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB054Mu01 | Recombinant Apoptotic Peptidase Activating Factor 1 (APAF1) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB054Mu02 | Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) | WB; IHC; ICC; IP. |
| PAB054Mu01 | Polyclonal Antibody to Apoptotic Peptidase Activating Factor 1 (APAF1) | WB; IHC; IF |
| SEB054Mu | ELISA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB054Mu | CLIA Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB054Mu | Multiplex Assay Kit for Apoptotic Peptidase Activating Factor 1 (APAF1) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |