Anti-Tubulin Beta (TUBb) Monoclonal Antibody
TUB-B; TUBB5; TUBB1; M40
- Product No.CAB870Mi22
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat) Same name, Different species.
- PurificationProtein A + Protein G affinity chromatography
- LabelNone
- Buffer FormulationPBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- TraitsLiquid
- Concentration0.65mg/mL
- Organism Species MoreHamster (Chinese hamster), Oryctolagus cuniculus (Rabbit), Canis familiaris; Canine (Dog), Sus scrofa; Porcine (Pig), Bos taurus; Bovine (Cattle), Capra hircus; Caprine (Goat), Ovis aries; Ovine (Sheep), Chicken (Gallus)
- ApplicationsLoading Control of WB
- Download Instruction Manual
- UOM 20µl100µl 200µl 1ml 10ml
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FOB
US$ 119
For more details, please contact local distributors!US$ 279
For more details, please contact local distributors! US$ 398
For more details, please contact local distributors! US$ 995
For more details, please contact local distributors! US$ 3980
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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DAB staining on IHC-P; Sample: Human Breast cancer Tissue; Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Colon Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cerebrum Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Cardiac Muscle Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lymphoma Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Lung Tissue;
Primary Ab: 10µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
FITC staining on IF;
Sample: Human MCF7 cell;
Primary Ab: 40µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 1µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18) -
FITC staining on IF;
Sample: Human HepG2 cell;
Primary Ab: 20µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 2µg/ml FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu18)
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Figure. Western Blot Sample: Recombinant TUBb
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Western Blot analysis of TUBb in various cell lines
Lane1: HL60 whole cell lysate
Lane2: THP-1 whole cell lysate
Lane3: MDA-MB-435S whole cell lysate
Lane4: T-47D whole cell lysate
Lysates/proteins at 20µg per lane.
Primary Ab: anti-TUBb antibody (CAB870Mi22) at 0.3ng/ml
Secondary Ab: HRP-conjugated Rabbit anti-mouse antibody (SAA544Mu09) at 1/10000 dilution
Developed using the ECL technique.
Calculated MW: 50KD
Observed MW: 50KD
Exposure time: 1 min -
Western Blot; Sample: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Lane3: Canine Kidney lysate; Lane4: Gallus Cerebrum lysate; Lane5: Gallus Kidney lysate; Lane6: Bovine Cerebrum lysate; Lane7: Bovine Kidney lysate; Lane8: Rabbit Kidney lysate; Lane9: Cavia Kidney lysate; Lane10: Caprine Kidney lysate
Primary Ab: 1µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: DU145 cell lysate; Lane2: HL60 cell lysate; Lane3: THP1 cell lysate; Lane4: OS-RC-2 cell lysate; Lane5: HEC-1B cell lysate; Lane6: MDA-MB-435S cell lysate; Lane7: T-47D cell lysate
Primary Ab: 0.5µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Heart lysate; Lane3: Mouse Cerebellum lysate; Lane4: Porcine Cerebrum lysate; Lane5: Bovine Kidney lysate; Lane6: Rabbit Pancreas lysate
Primary Ab: 0.0003µg/ml Mouse Anti-Multi-species TUBb Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Anti-Tubulin Beta (TUBb) Monoclonal Antibody
The antibody is a mouse monoclonal antibody raised against TUBb. It has been selected for its ability to recognize TUBb in immunohistochemical staining and western blotting.
USAGE of the Anti-Tubulin Beta (TUBb) Monoclonal Antibody
Western blotting: 0.0003-0.1ug/ml
Optimal working dilutions must be determined by end user.
STORAGE of the of the Anti-Tubulin Beta (TUBb) Monoclonal Antibody
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Anti-Tubulin Beta (TUBb) Monoclonal Antibody
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
INCREMENT SERVICES
Antibody Labeling Customized Service
Protein A/G Purification Column
Staining Solution for Cells and Tissue
Positive Control for Antibody
Tissue/Sections Customized Service
Phosphorylated Antibody Customized Service
Western Blot (WB) Experiment Service
Immunohistochemistry (IHC) Experiment Service
Immunocytochemistry (ICC) Experiment Service
Flow Cytometry (FCM) Experiment Service
Immunoprecipitation (IP) Experiment Service
Immunofluorescence (IF) Experiment Service
Buffer
DAB Chromogen Kit
SABC Kit
Long-arm Biotin Labeling Kit
Real Time PCR Experimental Service
Related products
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