Active Tumor Necrosis Factor Alpha (TNFa)

DIF; TNF-A; TNFSF2; Cachectin; Tumor Necrosis Factor Ligand Superfamily Member 2

  • Active Tumor Necrosis Factor Alpha (TNFa)Packages (Simulation)
  • Active Tumor Necrosis Factor Alpha (TNFa)Packages (Simulation)
  • Active Tumor Necrosis Factor Alpha (TNFa)Gene sequencing
  • APA133Mu01.jpgFigure. SDS-PAGE
  • Active Tumor Necrosis Factor Alpha (TNFa)Western Blot, Sample: APA133Mu01; Antibody: PAA133Mu01
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Tumor Necrosis Factor Alpha (TNFa)

Figure 1. Cell apoptosis of A549 cells after stimulated by TNFa.
TNFa, being an endogenous pyrogen, is able to induce fever, apoptotic cell death, inflammation and inhibit tumorigenesis. As reported, TNFa could inhibit the proliferation and induce apoptosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β in cell supernatant was detected by ELISA. Cell apoptosis of A549 after incubation of 48h was shown in Figure 1. (A)A549 cells cultured in DMEM, stimulated with 1ng/mL TNFa for 48h;
(B)A549 cells cultured in DMEM, stimulated with 10ng/mL TNFa for 48h;
A549 cells cultured in DMEM for 48h.

Table 1. ELISA detection of IL-1β expression from A549 cells by TNFa.
Effect of TNFa on the expression of IL-1β is shown in Table 1.

USAGE of the Active Tumor Necrosis Factor Alpha (TNFa)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Tumor Necrosis Factor Alpha (TNFa)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Tumor Necrosis Factor Alpha (TNFa)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Mus musculus (Mouse)Applications (RESEARCH USE ONLY!)
APA133Mu01Active Tumor Necrosis Factor Alpha (TNFa)Cell culture; Activity Assays.
APA133Mu61Active Tumor Necrosis Factor Alpha (TNFa)Cell culture; Activity Assays.
RPA133Mu02Recombinant Tumor Necrosis Factor Alpha (TNFa)Positive Control; Immunogen; SDS-PAGE; WB.
EPA133Mu61Eukaryotic Tumor Necrosis Factor Alpha (TNFa)Positive Control; Immunogen; SDS-PAGE; WB.
RPA133Mu01Recombinant Tumor Necrosis Factor Alpha (TNFa)Positive Control; Immunogen; SDS-PAGE; WB.
PAA133Mu01Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC; IP.
PAA133Mu02Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC; IP.
PAA133Mu06Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC; IP.
LAA133Mu71Biotin-Linked Polyclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC.
MAA133Mu21Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)WB; IHC; ICC; IP.
SEA133MuELISA Kit for Tumor Necrosis Factor Alpha (TNFa)Enzyme-linked immunosorbent assay for Antigen Detection.
MEA133MuMini Samples ELISA Kit for Tumor Necrosis Factor Alpha (TNFa)Enzyme-linked immunosorbent assay for Antigen Detection.
SCA133MuCLIA Kit for Tumor Necrosis Factor Alpha (TNFa)Chemiluminescent immunoassay for Antigen Detection.
KSA133Mu01ELISA Kit DIY Materials for Tumor Necrosis Factor Alpha (TNFa)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK