Active Tissue Factor (TF)

CD142; FIII; F3; TFA; Thromboplastin; Coagulation Factor III

  • Active Tissue Factor (TF)Packages (Simulation)
  • Active Tissue Factor (TF)Packages (Simulation)
  • APA524Ra01.jpgSDS-PAGE
  • Active Tissue Factor (TF)Figure. Western Blot; Sample: Recombinant Tissue Factor, Rat.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Tissue Factor (TF)

TF (Tissue factor) also known for platelet tissue factor, factor III, thromboplastin, or CD142, is a cell surface glyprotein present in subendothelial tissue and leukocytes. TF is necessary for the initiation of thrombin formation from the zymogen prothrombin. It has been proven that EGF can bind with the TF on the hemangioendotheliocytes. Thus a binding ELISA assay was conducted to detect the interaction of recombinant rat TF and recombinant rat EGF. Briefly, TF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL TF were then transferred to EGF-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-TF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of TF and EGF was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Tissue Factor (TF)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Tissue Factor (TF)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Tissue Factor (TF)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications (RESEARCH USE ONLY!)
RPA524Ra01Recombinant Tissue Factor (TF)Positive Control; Immunogen; SDS-PAGE; WB.
APA524Ra01Active Tissue Factor (TF)Cell culture; Activity Assays.
PAA524Ra01Polyclonal Antibody to Tissue Factor (TF)WB; IHC; ICC; IP.
LAA524Ra81FITC-Linked Polyclonal Antibody to Tissue Factor (TF)WB; IHC; ICC; IF.
LAA524Ra71Biotin-Linked Polyclonal Antibody to Tissue Factor (TF)WB; IHC; ICC.
MAA524Ra21Monoclonal Antibody to Tissue Factor (TF)WB; IHC; ICC; IP.
SEA524RaELISA Kit for Tissue Factor (TF)Enzyme-linked immunosorbent assay for Antigen Detection.