Active Plasminogen (Plg)

PL; Plasmin; Activation peptide; Angiostatin

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
  • Active Plasminogen (Plg)Packages (Simulation)
  • Active Plasminogen (Plg)Packages (Simulation)
  • Active Plasminogen (Plg)Gene sequencing
  • APB236Ra01.jpgSDS-PAGE
  • Active Plasminogen (Plg)Figure. Western Blot; Sample: Recombinant Plg, Rat.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST

Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

GIVEAWAYS

Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications
RPB236Ra01Recombinant Plasminogen (Plg)Positive Control; Immunogen; SDS-PAGE; WB.
APB236Ra01Active Plasminogen (Plg)Cell culture; Activity Assays.
PAB236Ra01Polyclonal Antibody to Plasminogen (Plg)WB; IHC; ICC; IP.
MAB236Ra21Monoclonal Antibody to Plasminogen (Plg)WB; IHC; ICC; IP.
SEB236RaELISA Kit for Plasminogen (Plg)Enzyme-linked immunosorbent assay for Antigen Detection.
KSB236Ra01ELISA Kit DIY Materials for Plasminogen (Plg)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK