Active Plasminogen (Plg)

PL; Plasmin; Activation peptide; Angiostatin

  • Active Plasminogen (Plg)Packages (Simulation)
  • Active Plasminogen (Plg)Packages (Simulation)
  • Active Plasminogen (Plg)Gene sequencing
  • APB236Ra01.jpgSDS-PAGE
  • Active Plasminogen (Plg)Figure. Western Blot; Sample: Recombinant Plg, Rat.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Plasminogen (Plg)

Plasminogen (Plg) can be converted into active plasmin by tissue plasminogen activator (tPA), urokinase plasminogen activator (uPA), kallikrein, and factor XII (Hageman factor). Plasmin can dissolve fibrin blood clots, act on many other processes such as embryonic development, tissue remodeling, inflammation and tumor invasion. Plasmint also can activates collagenases, weakens the walls of the Graafian follicle, cleaves fibrin, fibronectin, thrombospondin, laminin, and von Willebrand factor. Besides, Actin Beta (ACTb) has been identified as an interactor of Plg, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat Plg and recombinant rat ACTb. Briefly, Plg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Plg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Plg and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Plasminogen (Plg)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Plasminogen (Plg)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Plasminogen (Plg)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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