Active Growth Differentiation Factor 15 (GDF15)

PDF; MIC1; NAG-1; PLAB; PTGFB; Macrophage inhibitory cytokine 1; NSAID-activated gene 1 protein; Placental bone morphogenetic protein; Prostate differentiation factor

  • Active Growth Differentiation Factor 15 (GDF15) Packages (Simulation)
  • Active Growth Differentiation Factor 15 (GDF15) Packages (Simulation)
  • APC034Hu62.jpg SDS-PAGE
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Growth Differentiation Factor 15 (GDF15)

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1), is a divergent member of the Transforming Growth Factor beta (TGF-beta ) superfamily. Human GDF-15 shares 66% and 68% amino acid sequence identity with the rat and mouse proteins, respectively. GDF-15 is highly expressed in placenta and brain, and it is expressed at lower levels in kidney, pancreas, prostate, and colon. Similar to other TGF-beta family proteins, GDF-15 is synthesized as a large precursor protein that is cleaved at a dibasic cleavage site (RxxR) to release the mature protein. Biologically active GDF-15 is a disulfide-linked homodimer of the mature protein. GDF 15 has been shown to have various functions, including inhibition of Tumor Necrosis Factor alpha (TNF-alpha ) production from lipopolysaccharide-stimulated macrophages and the induction of cartilage formation. A functional ELISA assay was conducted to detect the interaction of recombinant human GDF-15 and recombinant human Transforming Growth Factor Beta Receptor II (TGFbR2). Briefly, biotin-linked GDF-15 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to TGFbR2-coated microtiter wells and incubated for 1h at 37 ℃. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 ℃. Finally, add 50 µl stop solution to the wells and read at 450 nm immediately. The binding activity of GDF-15 and TGFbR2 was shown in Figure 1, the EC50 for this effect is 7.002 ug/mL.

USAGE of the Active Growth Differentiation Factor 15 (GDF15)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Growth Differentiation Factor 15 (GDF15)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Growth Differentiation Factor 15 (GDF15)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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