Active Galectin 9 (GAL9)
LGALS9; Ecalectin; Lectin,Galactoside-Binding Soluble 9; Tumor antigen HOM-HD-21
- Product No.APA309Hu61
- Organism SpeciesHomo sapiens (Human) Same name, Different species.
- Buffer FormulationPBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point9.3
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
-
FOB
US$ 220
For more details, please contact local distributors!US$ 550
For more details, please contact local distributors! US$ 1100
For more details, please contact local distributors! US$ 3300
For more details, please contact local distributors! US$ 8250
For more details, please contact local distributors!
ACTIVITY TEST of the Active Galectin 9 (GAL9)
Galectin 9 (GAL9) is a member of the β-galactoside–binding galectin family. Galectin-9 is found outside of cells and may be exported by non-classical pathways. Galectin 9 exhibits a variety of biological activities, the majority of which have focused on its immunomodulatory role toward lymphocytes, were it shows specific interactions with TIM-3, and can negatively regulate Th1 type immunity. It also can agglutinate red blood. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant human GAL9 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL9 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL9, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results were shown in Figure 1. It was obvious that the minimal effective concentration of GAL9 is 3.125 μg/mL.
Figure 2. The hemagglutination assay of GAL9 in V- bottom shaped 96-well microtiter plate.
USAGE of the Active Galectin 9 (GAL9)
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Galectin 9 (GAL9)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Galectin 9 (GAL9)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
INCREMENT SERVICES
BCA Protein Quantification Kit
Molecular Mass Marker for Protein
Monoclonal Antibody Customized Service
Polyclonal Antibody Customized Service
Protein Activity Test Experiment Service
Electrophoretic Mobility Shift Assay (EMSA) Experiment Service
Buffer
Lentivirus Packaging Experiment Service
Adenovirus Packaging Experiment Service
Real Time PCR Experimental Service
Spike RBD Protein (S-RBD)
Protein G
Protein A
Related products
Catalog No. | Organism species: Homo sapiens (Human) | Applications (RESEARCH USE ONLY!) |
RPA309Hu01 | Recombinant Galectin 9 (GAL9) | Positive Control; Immunogen; SDS-PAGE; WB. |
APA309Hu01 | Active Galectin 9 (GAL9) | Cell culture; Activity Assays. |
APA309Hu61 | Active Galectin 9 (GAL9) | Cell culture; Activity Assays. |
EPA309Hu61 | Eukaryotic Galectin 9 (GAL9) | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA309Hu01 | Polyclonal Antibody to Galectin 9 (GAL9) | WB; IHC |
MAA309Hu22 | Monoclonal Antibody to Galectin 9 (GAL9) | WB; IHC; ICC; IP. |
SEA309Hu | ELISA Kit for Galectin 9 (GAL9) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SCA309Hu | CLIA Kit for Galectin 9 (GAL9) | Chemiluminescent immunoassay for Antigen Detection. |
LMA309Hu | Multiplex Assay Kit for Galectin 9 (GAL9) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |