Active Dickkopf Related Protein 1 (DKK1)

SK; Dickkopf Homolog 1(Xenopus Laevis)

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
  • Active Dickkopf Related Protein 1 (DKK1)Packages (Simulation)
  • Active Dickkopf Related Protein 1 (DKK1)Packages (Simulation)
  • Active Dickkopf Related Protein 1 (DKK1)Gene sequencing
  • APA741Hu01.jpgSDS-PAGE
  • Active Dickkopf Related Protein 1 (DKK1)Figure. Western Blot; Sample: Recombinant DKK1, Human.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST

Dickkopf-related protein 1 (DKK1) is a protein which is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the Wnt signaling pathway. Dickkopf WNT signaling pathway inhibitor 1 (Dkk1) is a protein-coding gene that acts from the anterior visceral endoderm. The dickkopf protein encoded by DKK1 is an antagonistic inhibitor of the WNT signaling pathway that acts by isolating the LRP6 co-receptor so that it cannot aid in activating the WNT signaling pathway. DKK1 was also demonstrated to antagonize the Wnt/β-catenin pathway via a reduction in β-catenin and an increase in OCT4 expression. Besides, Low Density Lipoprotein Receptor Related Protein 6 (LRP6) has been identified as an interactor of DKK1 thus a binding ELISA assay was conducted to detect the interaction of recombinant human DKK1 and recombinant human LRP6. Briefly, DKK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to LRP6-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DKK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DKK1 and LRP6 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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