Active Defensin Beta 2 (DEFb2)

B-DF2; DEFB2; BD2; DEF-B2; HBD2; DEFB102; HBD-2; SAP1; DEFB4A; DEFB4; Skin-Antimicrobial Peptide 1

  • Active Defensin Beta 2 (DEFb2)Packages (Simulation)
  • Active Defensin Beta 2 (DEFb2)Packages (Simulation)
  • Active Defensin Beta 2 (DEFb2)Figure. Gene Sequencing (Extract)
  • APA072Hu01.pngFigure. SDS-PAGE
  • Active Defensin Beta 2 (DEFb2)Figure. Western Blot
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Defensin Beta 2 (DEFb2)

Figure. The binding activity of DEFb2 with KCNA3.
Defensin Beta 2 (DEFb2) known as skin-antimicrobial peptide 1 (SAP1) is a cysteine-rich cationic low molecular weight antimicrobial peptide. Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family are highly similar in protein sequence. DEFb2 is produced by a number of epithelial cells and exhibits potent antimicrobial activity against Gram-negative bacteria and Candida, but not Gram-positive S. aureus. Besides, potassium voltage-gated channel, shaker-related subfamily, member 3 (KCNA3) has been identified as an interactor of DEFb2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human DEFb2 and recombinant human KCNA3. Briefly, DEFb2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to KCNA3-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-DEFb2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of DEFb2 and KCNA3 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Defensin Beta 2 (DEFb2)

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Defensin Beta 2 (DEFb2)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Defensin Beta 2 (DEFb2)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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