Active Arylsulfatase F (ARSF)
- Product No.APG613Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.9
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 368
For more details, please contact local distributors!US$ 920
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant ARSF, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Arylsulfatase F (ARSF)
ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Arylsulfatase F (ARSF)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Arylsulfatase F (ARSF)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Arylsulfatase F (ARSF)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Homo sapiens (Human)||Applications (RESEARCH USE ONLY!)|
|RPG613Hu01||Recombinant Arylsulfatase F (ARSF)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APG613Hu01||Active Arylsulfatase F (ARSF)||Cell culture; Activity Assays.|
|PAG613Hu01||Polyclonal Antibody to Arylsulfatase F (ARSF)||WB; IHC; ICC; IP.|
|MAG613Hu21||Monoclonal Antibody to Arylsulfatase F (ARSF)||WB; IHC; ICC; IP.|
|SEG613Hu||ELISA Kit for Arylsulfatase F (ARSF)||Enzyme-linked immunosorbent assay for Antigen Detection.|