Active Arylsulfatase F (ARSF)

ASF

  • Active Arylsulfatase F (ARSF)Packages (Simulation)
  • Active Arylsulfatase F (ARSF)Packages (Simulation)
  • APG613Hu01.jpgSDS-PAGE
  • Active Arylsulfatase F (ARSF)Figure. Western Blot; Sample: Recombinant ARSF, Human.
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

ACTIVITY TEST of the Active Arylsulfatase F (ARSF)

ARSF (Arylsulfatase F) is a member of the sulfatase gene family, and more specifically, the arylsulfatase subfamily. Sulfatases are essential for the correct composition of bone and cartilage matrix. The activity of this protein, unlike that of arylsulfatase E, is not inhibited by warfarin. Besides, EP300 (E1A binding protein p300) has been identified as an interactor of ARSF, thus a binding ELISA assay was conducted to detect the interaction of recombinant human ARSF and recombinant human EP300. Briefly, ARSF were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to EP300-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-ARSF pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of ARSF and EP300 was shown in Figure 1, and this effect was in a dose dependent manner.

USAGE of the Active Arylsulfatase F (ARSF)

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE of the Active Arylsulfatase F (ARSF)

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY of the Active Arylsulfatase F (ARSF)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
RPG613Hu01Recombinant Arylsulfatase F (ARSF)Positive Control; Immunogen; SDS-PAGE; WB.
APG613Hu01Active Arylsulfatase F (ARSF)Cell culture; Activity Assays.
PAG613Hu01Polyclonal Antibody to Arylsulfatase F (ARSF)WB; IHC; ICC; IP.
MAG613Hu21Monoclonal Antibody to Arylsulfatase F (ARSF)WB; IHC; ICC; IP.
SEG613HuELISA Kit for Arylsulfatase F (ARSF)Enzyme-linked immunosorbent assay for Antigen Detection.
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