Active Arginase (ARG)
ARG1; Arginase I; Liver Arginase
- Product No.APB120Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Buffer Formulation20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point6.6
- ApplicationsCell culture; Activity Assays.
- Download Instruction Manual
- UOM 10µg50µg 200µg 1mg 5mg
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- Packages (Simulation)
- Packages (Simulation)
- Figure. Western Blot; Sample: Recombinant Arg, Mouse.
- ISO9001: 2008, ISO13485: 2003 Registered
ACTIVITY TEST of the Active Arginase (ARG)
Arginase (Arg) is an enzyme that catalyzes the degradation of arginine to produce urea and omithine, which is crucial in the urea cycle. In most mammals, two isozymes of this enzyme exist; the first, Arginase I, functions in the urea cycle, and is located primarily in the cytoplasm of the liver. The second isozyme, Arginase II, has been implicated in the regulation of the arginine/ornithine concentrations in the cell. Besides, Ubiquitin Carboxyl Terminal Hydrolase L5 (UCHL5) has been identified as an interactor of Arg, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse Arg and recombinant mouse UCHL5. Briefly, Arg were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to UCHL5-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-Arg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of Arg and UCHL5 was shown in Figure 1, and this effect was in a dose dependent manner.
USAGE of the Active Arginase (ARG)
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE of the Active Arginase (ARG)
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY of the Active Arginase (ARG)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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|Catalog No.||Organism species: Mus musculus (Mouse)||Applications (RESEARCH USE ONLY!)|
|RPB120Mu02||Recombinant Arginase (ARG)||Positive Control; Immunogen; SDS-PAGE; WB.|
|RPB120Mu01||Recombinant Arginase (ARG)||Positive Control; Immunogen; SDS-PAGE; WB.|
|APB120Mu01||Active Arginase (ARG)||Cell culture; Activity Assays.|
|PAB120Mu01||Polyclonal Antibody to Arginase (ARG)||WB; IHC; ICC; IP.|
|PAB120Mu02||Polyclonal Antibody to Arginase (ARG)||WB; IHC; ICC; IP.|
|LAB120Mu81||FITC-Linked Polyclonal Antibody to Arginase (ARG)||WB; IHC; ICC; IF.|
|LAB120Mu71||Biotin-Linked Polyclonal Antibody to Arginase (ARG)||WB; IHC; ICC.|
|SEB120Mu||ELISA Kit for Arginase (ARG)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|MEB120Mu||Mini Samples ELISA Kit for Arginase (ARG)||Enzyme-linked immunosorbent assay for Antigen Detection.|
|KSB120Mu01||ELISA Kit DIY Materials for Arginase (ARG)||Main materials for "Do It (ELISA Kit) Yourself".|