Active Adenine Phosphoribosyltransferase (APRT)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
- Product No.APC310Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- Brand OwnerCLOUD-CLONE CORP.(CCC, USA)
- ManufacturerCLOUD-CLONE CORP.(CCC, WUH)
- Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 97%
- Isoelectric Point6.8
- ApplicationsCell culture; Activity Assays.
- DownloadInstruction Manual
- FOBUS$ 380
For negotiated price and more details, please contact local distributors!US$ 950
For negotiated price and more details, please contact local distributors!US$ 1900
For negotiated price and more details, please contact local distributors!US$ 5700
For negotiated price and more details, please contact local distributors!US$ 142500
For negotiated price and more details, please contact local distributors!
- Packages (Simulation)
- Packages (Simulation)
- Gene sequencing
- Figure. Western Blot; Sample: Recombinant APRT, Human.
- ISO9001: 2008, ISO13485: 2003 Registered
Adenine Phosphoribosyltransferase (APRT) is an enzyme involved in the purine nucleotide salvage pathway. It functions as a catalyst in the reaction between adenine and phosphoribosyl pyrophosphate (PRPP) to form AMP. Besides, Vascular Cell Adhesion Molecule 1 (VCAM1) has been identified as an interactor of APRT, thus a binding ELISA assay was conducted to detect the interaction of recombinant human APRT and recombinant human VCAM1. Briefly, APRT were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to VCAM1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-APRT pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of of APRT and VCAM1 was shown in Figure 1, and this effect was in a dose dependent manner.
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
|Catalog No.||Organism species: Homo sapiens (Human)||Applications|
|APC310Hu01||Active Adenine Phosphoribosyltransferase (APRT)||Cell culture; Activity Assays.|
|RPC310Hu01||Recombinant Adenine Phosphoribosyltransferase (APRT)||Positive Control; Immunogen; SDS-PAGE; WB.|
|PAC310Hu01||Polyclonal Antibody to Adenine Phosphoribosyltransferase (APRT)||WB; IHC; ICC; IP.|
|MAC310Hu22||Monoclonal Antibody to Adenine Phosphoribosyltransferase (APRT)||WB; IHC; ICC; IP.|
|SEC310Hu||ELISA Kit for Adenine Phosphoribosyltransferase (APRT)||Enzyme-linked immunosorbent assay for Antigen Detection.|