Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

HMGB1; HMG3; SBP1; Sulfoglucuronyl Carbohydrate Binding Protein; Amphoterin; High Mobility Group Box 1 Protein

  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Packages (Simulation)
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Packages (Simulation)
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Figure. DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue.
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Figure. DAB staining on IHC-P;
    Samples: Human Lung Cancer Tissue.
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Figure. DAB staining on IHC-P;
    Samples: Human Skin Cancer Tissue.
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Figure. DAB staining on IHC-P;
    Samples: Human Rectum Cancer Tissue.
  • PAA399Hu02.jpgFigure. Western Blot; Sample: Recombinant HMG1, Human.
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Figure. Western Blot; Sample: Lane1: Human Hela Cells; Lane2: Human HepG2 Cells; Lane3: Human Jurkat Cells.
  • Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)Knockout Varification:
    Lane 1: Wild-type Hela cell lysate;
    Lane 2: HMG1 knockout Hela cell lysate;
    Predicted MW: 25kd
    Observed MW: 30kd
    Primary Ab: 5µg/ml Rabbit Anti-Human HMG1 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

The antibody is a rabbit polyclonal antibody raised against HMG1. It has been selected for its ability to recognize HMG1 in immunohistochemical staining and western blotting.

USAGE of the Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.

STORAGE of the of the Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

STABILITY of the Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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