Polyclonal Antibody to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
- Product No.PAB246Hu01
- Organism SpeciesHomo sapiens (Human)Same name, Different species.
- SourcePolyclonal antibody preparation
- HostRabbit
- Potencyn/a
- Ig TypeIgG
- PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- LabelNone
- ImmunogenRPB246Hu01-Recombinant Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
- Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
- TraitsLiquid
- Concentration500µg/mL
- Organism Species Moren/a
- ApplicationsWB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies. - DownloadInstruction Manual
- UOM20µl100µl200µl1ml10ml
- FOBUS$ 100
For more details, please contact local distributors!US$ 232
For more details, please contact local distributors!US$ 332
For more details, please contact local distributors!US$ 830
For more details, please contact local distributors!US$ 3320
For more details, please contact local distributors!
Packages (Simulation)- Packages (Simulation)
- DAB staining on IHC-P; Samples: Human Prostate Tissue; Primary Ab: 10µg/ml Rabbit Anti-Human CYFRA2
- DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CYFRA21-1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CYFRA21-1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - DAB staining on IHC-P;
Samples: Human Colorectal cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CYFRA21-1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) - DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 10µg/ml Rabbit Anti-Human CYFRA21-1 Antibody
Second Ab: 2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
- Figure. Western Blot; Sample: Recombinant CYFRA21-1, Human.
- Figure. Western Blot; Sample: Lane1: Human Serum; Lane2: Human MCF-7 Cells; Lane3: Human A431 Cells; Lane4: Human HepG2 Cells.
- Knockout Varification:
Lane 1: Wild-type MCF7 cell lysate;
Lane 2: CYFRA21-1 knockout MCF7 cell lysate;
Predicted MW: 44kd
Observed MW: 50kd
Primary Ab: 5µg/ml Rabbit Anti-Human CYFRA21-1 Antibody
Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
- ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the Polyclonal Antibody to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
The antibody is a rabbit polyclonal antibody raised against CYFRA21-1. It has been selected for its ability to recognize CYFRA21-1 in immunohistochemical staining and western blotting.
USAGE of the Polyclonal Antibody to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
STORAGE of the of the Polyclonal Antibody to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY of the Polyclonal Antibody to Cytokeratin Fragment Antigen 21-1 (CYFRA21-1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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