Mouse Model for Tumor Transplantation (TT)
Transplanted tumors;TE-1;HepG2;HepG2-luc;SK-MES-1;LL/2-luc-M38
- Product No.DSI530Mu02
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Prototype SpeciesHuman
- SourceInduced by HepG2-luc in situ tumor formation
- Model Animal StrainsBalb/ -nude Mice(SPF), healthy, male, age:5 weeks, body weight:18g~20g.
- Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
- Modeling Period4-6 weeks
- Modeling Method1. Cell line: HepG2-luc human hepatoma cell line
2. Liver tumor in situ formation model :
2.1 The HepG2-luc cells cultured in logarithmic growth phase were digested and centrifuged, and the resulting approximately 5 x 10e6 cells were dissolved in 0.1 mL DMEM culture medium.
2.2 Use 3% pentobarbital to anesthetize mice, laparotomy to expose the liver, the prepared cells were injected into the liver of nude mice, with 5-0 silk suture wounds, povidone iodine to disinfect wounds, the nude mice were placed in a heating pad, stay awake after normal diet feeding.
3. Fluorescence in vivo imaging:
For in vivo fluorescence imaging detection of tumor in nude mice, mice were anesthetized with 3% pentobarbital sodium used before imaging, by intraperitoneal injection of 3mg luciferin substrate, the substrate after the injection of 20~30 minutes after the in vivo fluorescence imaging detection. - ApplicationsDisease Model for HepG2-luc in situ tumor formation
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US$ 280
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Model Evaluation of the Mouse Model for Tumor Transplantation (TT)
Pathological Results of the Mouse Model for Tumor Transplantation (TT)
Cytokines Level of the Mouse Model for Tumor Transplantation (TT)
Statistical Analysis of the Mouse Model for Tumor Transplantation (TT)
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
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Nitric Oxide Assay Kit (A013-2)
Total Anti-Oxidative Capability Assay Kit(A015-2)
Total Anti-Oxidative Capability Assay Kit (A015-1)
Superoxide Dismutase Assay Kit
Fructose Assay Kit (A085)
Citric Acid Assay Kit (A128 )
Catalase Assay Kit
Malondialdehyde Assay Kit
Glutathione S-Transferase Assay Kit
Microscale Reduced Glutathione assay kit
Glutathione Reductase Activity Coefficient Assay Kit
Angiotensin Converting Enzyme Kit
Glutathione Peroxidase (GSH-PX) Assay Kit
Cloud-Clone Multiplex assay kits
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