Mouse Model for Skin Photoaging (SP)

  • Product No.DSI672Mu01
  • Organism SpeciesMus musculus (Mouse) Same name, Different species.
  • Prototype SpeciesHuman
  • SourceInduced by ultraviolet ray（UVA+UVB）
  • Model Animal StrainsICR Mice(SPF), healthy, male, age: 6~8weeks.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
  • Modeling MethodMale ICR mice, aged about 6 weeks. Mice were fed at normal temperature (23±2℃) humidity (55%±10%) and light (12 hours of light / 12 hours of darkness, no ULTRAVIOLET radiation). After feeding for one week, when the mice were acclimated to the environment, a depilation area of more than 2 cm x3 cm was shaved on the back of the mice with 8% sodium sulfide.

    Ultraviolet irradiation method: 2 UVB tubes and 4 UVA tubes (40W, Beijing Institute of Electric Light Source) are placed in a self-made uv lighting box as uv lighting source. The radiation intensity of UVA and UVB was measured by uV-B ultraviolet irradiometer and UV-A ultraviolet irradiometer. Before building, UV lamp preheating 10 min, after waiting for light source stability put rats in homemade rat cage (make no climbing, standing) between mice, and into the box as in cage and UV lamp are 30 cm apart, according to the forecast of the minimal erythema dose (MED) and light intensity adjustment according to time, using exhaust fan to reduce body temperature during irradiation. In the first week, the dose of one MED was irradiated 3 times per week. In the following week, the dose increased by 1 MED per week compared with the previous week until 4 MED in the fourth week, and then 4 MED was maintained until the end of the experiment in the eighth week. In the model group, the skin on the back of mice showed obvious signs of photoaging, such as thickening, deep wrinkles and relaxation, indicating that the modeling of mouse skin photoaging was successful.

    MED determination method: The back skin of pre-experimental mice was irradiated with uv radiation doses of 30mJ/cm2, 60mJ/cm2, 90mJ/cm2, 120mJ/cm2, 150mJ/cm2 and 180mJ/cm2, respectively. The minimum erythema dose (MED) was required to observe the visible erythema on the back skin of mice 24h after irradiation.
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  • Mouse Model for Skin Photoaging (SP) Packages (Simulation)
  • Mouse Model for Skin Photoaging (SP) Packages (Simulation)
  • Mouse Model for Skin Photoaging (SP) Fig.HE staining of skin,NC=control，MC=modeling，PC=Model positive
  • DSI672Mu01.png Fig.Skin of mice,NC=control，MC=modeling，PC=Model+positive
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Model Evaluation of the Mouse Model for Skin Photoaging (SP)

1. Determination of minimum Erythema dose (MED) :

Choose 2 UVB tubes and 4 UVA tubes, before modeling, UV tube preheating 10 min, mouse cage and UV tube 30 cm apart (at this time the total irradiation of UVA measured by the irradiator is about 3.25 uW/cm2; The total irradiation amount of UVB is about 0.134 uW/cm2. This value fluctuates slightly).

Eight ICR mice were selected, the hair on the back of the mice was shaved with a hair shaver, and the remaining hair was removed with 8% sodium sulfide. Then four irradiation time points were set: The mice were irradiated for 10, 15, 20 and 30 min, with 2 mice at each time point. The mice were taken out at the irradiation time point and fed normally. The erythema on the back of the mice were observed on the second day. Mice irradiated for 10 and 15 minutes had no obvious erythema. Mice irradiated for 20 minutes showed mild erythema, while mice irradiated for 30 minutes showed obvious erythema. Irradiation for 20 minutes was selected as the minimum erythema dose (MED) obtained in the pre-test.

1 MED= (3.25uw /cm2+ 0.134uw /cm2) *20 min *60=4 mJ/cm2

2. The whole rat's back was photographed

Before sampling at week 4 and 8, photographs of the whole rat's back were taken to determine whether there were photoaging characteristics (deep wrinkles, relaxation, etc.).

3. Immune indexes: spleen and thymus indexes were determined

The mice were weighed and performed cervical dislocation, and the spleen and thymus were immediately removed from the mice and weighed immediately. Thymus and spleen indices are based on the following equation

Calculation: Thymus or spleen index (mg/g) = thymus or spleen weight/body weight.

TI and SI can be used as immune indexes.

Pathological Results of the Mouse Model for Skin Photoaging (SP)

Histological analysis

Skin samples were taken for histochemical study within 24h after final uv irradiation. Mouse skin samples were fixed in 4% buffered neutral formalin solution for 24h, embedded in paraffin, and sequentially sectioned.

Cytokines Level of the Mouse Model for Skin Photoaging (SP)

Antioxidant index

1. Skin antioxidant index analysis

Skin tissue from the back of mice (about 2cm × 3cm) was divided into 8 pieces on average. One piece was fixed with formalin for pathological examination. One piece was stored with RNA later at -80 degrees for sequencing; For the remaining 6 pieces, place them in a tube 2 at a time at -80 degrees).

2. Antioxidant activity in serum

At the end of the experiment, the mice fasted for 12 hours but were allowed to enter the water freely. Blood is collected from the eyeball into a centrifugal tube. Serum was obtained from the blood sample after centrifugation (7000 g, 4℃,15 min), then frozen and stored at 20℃ until analysis

Statistical Analysis of the Mouse Model for Skin Photoaging (SP)

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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