Mouse Model for Pulmonary Embolism (PE)
- Product No.DSI517Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Prototype SpeciesHuman
- SourceThrombin induced
- Model Animal StrainsBalb/c mice (SPF class), male, weeks in age:4weeks~6weeks, 20g~22g.
- Modeling GroupingRandomly divided into groups: Control group, Model group, Positive drug group and Test drug group, 15 mice per group.
- Modeling Period1~2 days
- Modeling Method1. 22g-26g mice, with 3% Pentobarbital Sodium, with 80mg/kg intraperitoneally anesthetized mice, mice in supine position, the limbs and head with tape fixed on anatomical plate, with 8% sodium sulfide to remove the neck hair, povidone iodine disinfection in preparation for surgery.
2. Incision on the middle of the neck, the separation of soft tissue and muscle, exposure to the left side of the jugular vein, inject
Thrombin solution (soluble in 0.9% normal saline, dose 20U/kg) to the internal jugular vein .
3. Clean up the operation field, suture the neck incision, stay awake and put mice back to clean cage, observe the state and the state of the mouse and record.
4. Sham operation is the same as that operation in modeling group,but the normal saline injected into the jugular vein.
5. After 24hrs, kill the mice, take the left lung tissue, 4% poly formaldehyde solution fixed, paraffin embedded; the rest of the lung tissue -80 degree preservation. - ApplicationsDisease Model
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- UOM Each case
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US$ 140
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Model Evaluation of the Mouse Model for Pulmonary Embolism (PE)
Peripheral blood platelet count:
collect 20ul mice peripheral blood , put in 380 μL (10g/L) ammonium oxalate dilution, after mixing and use blood cell counting plate platelet count.
Pathological Results of the Mouse Model for Pulmonary Embolism (PE)
Take the left lung, 4% poly formaldehyde fixed, and then dehydration, paraffin embedded, sliced (thickness of 5μm), HE staining.
A few hours after that alveolar exudation and hemorrhage, alveolar septal thickening, which a large number of inflammatory cell infiltration, visible large artery embolism, platelets and red blood cell aggregation and adhesion to around the embolus.
Cytokines Level of the Mouse Model for Pulmonary Embolism (PE)
Statistical Analysis of the Mouse Model for Pulmonary Embolism (PE)
SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.
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Immunohistochemistry (IHC) Experiment Service
Small Animal In Vivo Imaging Experiment Service
Small Animal Micro CT Imaging Experiment Service
Small Animal MRI Imaging Experiment Service
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Transmission Electron Microscopy (TEM) Experiment Service
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Nitric Oxide Assay Kit (A012)
Nitric Oxide Assay Kit (A013-2)
Total Anti-Oxidative Capability Assay Kit(A015-2)
Total Anti-Oxidative Capability Assay Kit (A015-1)
Superoxide Dismutase Assay Kit
Fructose Assay Kit (A085)
Citric Acid Assay Kit (A128 )
Catalase Assay Kit
Malondialdehyde Assay Kit
Glutathione S-Transferase Assay Kit
Microscale Reduced Glutathione assay kit
Glutathione Reductase Activity Coefficient Assay Kit
Angiotensin Converting Enzyme Kit
Glutathione Peroxidase (GSH-PX) Assay Kit
Cloud-Clone Multiplex assay kits
Related products
Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
DSI517Mu01 | Mouse Model for Pulmonary Embolism (PE) | Disease Model |
TSI517Mu07 | Mouse Lung Tissue of Pulmonary Embolism (PE) | Paraffin slides for pathologic research: IHC,IF and HE,Masson and other stainings |