Mouse Model for Cerebral Ischemia (CI)

Brain Ischemia; Cerebrovascular Ischemia; MCAO

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
    • Product No.DSI523Mu01
    • Organism SpeciesMus musculus (Mouse)Same name, Different species.
    • Brand OwnerCLOUD-CLONE CORP.(CCC, USA)
    • ManufacturerCLOUD-CLONE CORP.(CCC, WUH)
    • Prototype SpeciesHuman
    • SourceIschemia-Reperfusion with MCAO
    • Model Animal StrainsBalb/c Mice(SPF level), male, healthy, body weight 25g~30g
    • Modeling GroupingRandomly divided into groups: Control group, Model group, Positive drug group and Test drug group, 15 mice per group.
    • Modeling Period24 hours, 3 days or 7 days
    • Modeling Method1. 3% sodium pentobarbital (80 mg/kg), neck skin preparation, disinfection, insert the anus temperature probe, maintain the temperature within 37±0.5℃.
      2. Incision of the middle of the neck, exposure the right common carotid artery, internal carotid artery and external carotid artery. Using 7-0 silk (at 2mm distance from the common carotid artery bifurcation ) to ligate the external carotid artery at the far end of the heart, pierces another 7-0 silk in the external carotid artery , and hit a slipknot near the bifurcation of the common carotid artery.
      3. Use arterial clamps to clamp the common carotid artery. Make a small cut on the external carotid artery (1.5 mm distance from bifurcation of common carotid artery ), a root tip treated 0.18 mm diameter nylon line from the cut insertion into the internal carotid artery, and inward into the middle cerebral artery, nylon line insertion depth distance carotid artery bifurcation about 9±1mm.
      4. Ischemia 90 mins and pull the suture, a 7-0 silk ligates artery proximal, 5-0 silk suture wounds on the neck, povidone iodine disinfection wound, put mice in a heating pad, and feed on constant temperature raising box after mice awaked.
      5. 24hrs after operation, neurological function score is evaluated, and then the mice are injected intraperitoneally with 3% sodium pentobarbital, and the brains are stained with TTC and pathological staining.
    • ApplicationsDiesase model
    • Downloadn/a
    • FormatEach case
    • FOBUS$ 90
      For negotiated price and more details, please contact local distributors!
    • Mouse Model for Cerebral Ischemia (CI)Packages (Simulation)
    • Mouse Model for Cerebral Ischemia (CI)Packages (Simulation)
    • DSI523Mu01.pngFig.TTC staining for modeling of mice brain ischemia-reperfusion
    • CertificateISO9001: 2008, ISO13485: 2003 Registered

    Model Evaluation

    1.Neurological dysfunction score
    Longa and Bederson's 5 point systems,make a score on mice awaked from anaesthesia 24 hours later.
    0 points: no nerve injury symptoms
    1 points can not fully extend the forepaw
    2 points: to the opposite side
    3 points: to the side dump
    4 points: can not walk spontaneously, loss of consciousness
    2.TTC staining
    Take the brain and store at -20℃, 1% TTC (W/V), 37℃ water bath for TTC dissolved, the frozen brain tissue slices, placed in 10ml TTC solution, 37℃, 10min. Normal brain tissue staining is bright red, and the infarct area is pale.

    Pathological Results

    Take the brain, 4% poly formaldehyde solution fixed, after dehydration of sucrose solution, the OCT embedded to make the frozen section (slice 10um), Nissl staining and the staining resluts are used for the evaluation of infarct size.

    Cytokines Level

    Statistical Analysis

    SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison, P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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