High Sensitive ELISA Kit for Claudin 3 (CLDN3)

C7orf1; CPE-R2; CPETR2; HRVP1; RVP1; Clostridium Perfringens Enterotoxin Receptor 2; Ventral Prostate.1-Like Protein; CPE-Receptor 2

  • High Sensitive ELISA Kit for Claudin 3 (CLDN3) Packages (Simulation)
  • High Sensitive ELISA Kit for Claudin 3 (CLDN3) Packages (Simulation)
  • High Sensitive ELISA Kit for Claudin 3 (CLDN3) Results demonstration
  • HEF293Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

This assay has high sensitivity and excellent specificity for detection of High Sensitive Claudin 3 (CLDN3).
No significant cross-reactivity or interference between High Sensitive Claudin 3 (CLDN3) and analogues was observed.

Recovery of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

Matrices listed below were spiked with certain level of recombinant High Sensitive Claudin 3 (CLDN3) and the recovery rates were calculated by comparing the measured value to the expected amount of High Sensitive Claudin 3 (CLDN3) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 85-97 91
EDTA plasma(n=5) 97-104 101
heparin plasma(n=5) 95-104 99

Precision of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Claudin 3 (CLDN3) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Claudin 3 (CLDN3) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of High Sensitive Claudin 3 (CLDN3) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 94-105% 82-98% 92-104% 98-105%
EDTA plasma(n=5) 97-105% 95-103% 80-96% 86-102%
heparin plasma(n=5) 94-103% 97-104% 78-92% 79-95%

Stability of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the High Sensitive ELISA Kit for Claudin 3 (CLDN3)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Claudin 3 (CLDN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Claudin 3 (CLDN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Claudin 3 (CLDN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Claudin 3 (CLDN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPF293Hu01 Recombinant Claudin 3 (CLDN3) Positive Control; Immunogen; SDS-PAGE; WB.
CPF293Hu21 OVA Conjugated Claudin 3 (CLDN3) Immunogen; SDS-PAGE; WB.
PAF293Hu01 Polyclonal Antibody to Claudin 3 (CLDN3) WB; IHC; ICC; IP.
MAF293Hu21 Monoclonal Antibody to Claudin 3 (CLDN3) WB; IHC; ICC; IP.
SEF293Hu ELISA Kit for Claudin 3 (CLDN3) Enzyme-linked immunosorbent assay for Antigen Detection.
HEF293Hu High Sensitive ELISA Kit for Claudin 3 (CLDN3) Enzyme-linked immunosorbent assay for Antigen Detection.
LMF293Hu Multiplex Assay Kit for Claudin 3 (CLDN3) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
GUESTBOOK