ELISA Kit for Vitamin B6 (VB6)

Pyridoxine

  • ELISA Kit for Vitamin B6 (VB6) Packages (Simulation)
  • ELISA Kit for Vitamin B6 (VB6) Packages (Simulation)
  • ELISA Kit for Vitamin B6 (VB6) Results demonstration
  • CEA916Ge.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Vitamin B6 (VB6)

This assay has high sensitivity and excellent specificity for detection of Vitamin B6 (VB6).
No significant cross-reactivity or interference between Vitamin B6 (VB6) and analogues was observed.

Recovery of the ELISA Kit for Vitamin B6 (VB6)

Matrices listed below were spiked with certain level of Vitamin B6 (VB6) and the recovery rates were calculated by comparing the measured value to the expected amount of Vitamin B6 (VB6) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 81-88 84
EDTA plasma(n=5) 83-102 92
heparin plasma(n=5) 99-105 102

Precision of the ELISA Kit for Vitamin B6 (VB6)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Vitamin B6 (VB6) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Vitamin B6 (VB6) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Vitamin B6 (VB6)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Vitamin B6 (VB6) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 90-105% 78-93% 90-99% 84-98%
EDTA plasma(n=5) 90-104% 81-97% 97-104% 86-104%
heparin plasma(n=5) 80-95% 86-105% 81-104% 93-101%

Stability of the ELISA Kit for Vitamin B6 (VB6)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Vitamin B6 (VB6)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Vitamin B6 (VB6)

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Vitamin B6 (VB6) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Vitamin B6 (VB6) and unlabeled Vitamin B6 (VB6) (Standards or samples) with the pre-coated antibody specific to Vitamin B6 (VB6). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Vitamin B6 (VB6) in the sample.

Related products

Catalog No. Organism species: Pan-species (General) Applications (RESEARCH USE ONLY!)
CPA916Ge41 HSA Conjugated Vitamin B6 (VB6) Immunogen; SDS-PAGE; WB.
CPA916Ge11 BSA Conjugated Vitamin B6 (VB6) Immunogen; SDS-PAGE; WB.
CPA916Ge21 OVA Conjugated Vitamin B6 (VB6) Immunogen; SDS-PAGE; WB.
PAA916Ge01 Polyclonal Antibody to Vitamin B6 (VB6) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
LAA916Ge71 Biotin-Linked Polyclonal Antibody to Vitamin B6 (VB6) WB; IHC; ICC.
MAA916Ge21 Monoclonal Antibody to Vitamin B6 (VB6) ELISA, CLIA. / IHC-Fr, ICC, IP (predicted).
CEA916Ge ELISA Kit for Vitamin B6 (VB6) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA916Ge Multiplex Assay Kit for Vitamin B6 (VB6) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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