ELISA Kit for Albumin (ALB)

Serum albumin

  • ELISA Kit for Albumin (ALB)Packages (Simulation)
  • ELISA Kit for Albumin (ALB)Packages (Simulation)
  • ELISA Kit for Albumin (ALB)Results demonstration
  • CEB028Ra.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Albumin (ALB)

This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.

Recovery of the ELISA Kit for Albumin (ALB)

Matrices listed below were spiked with certain level of recombinantAlbumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-9584
EDTA plasma(n=5)92-10196
heparin plasma(n=5)90-9894

Precision of the ELISA Kit for Albumin (ALB)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Albumin (ALB)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)84-96%92-104%85-102%92-99%
EDTA plasma(n=5)82-105%97-105%94-102%97-105%
heparin plasma(n=5)90-99%92-101%86-96%83-94%

Stability of the ELISA Kit for Albumin (ALB)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Albumin (ALB)

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
    And then add 50µL prepared Detection Reagent A immediately.
    Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.

Test principle of the ELISA Kit for Albumin (ALB)

The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Albumin (ALB) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Albumin (ALB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications (RESEARCH USE ONLY!)
NPB028Ra01Native Albumin (ALB)Positive Control; Immunogen; SDS-PAGE; WB.
RPB028Ra01Recombinant Albumin (ALB)Positive Control; Immunogen; SDS-PAGE; WB.
PAB028Ra01Polyclonal Antibody to Albumin (ALB)WB; IHC; ICC; IP.
LAB028Ra81FITC-Linked Polyclonal Antibody to Albumin (ALB)WB; IHC; ICC; IF.
LAB028Ra71Biotin-Linked Polyclonal Antibody to Albumin (ALB)WB; IHC; ICC.
MAB028Ra21Monoclonal Antibody to Albumin (ALB)WB; IHC; ICC; IP.
CEB028RaELISA Kit for Albumin (ALB)Enzyme-linked immunosorbent assay for Antigen Detection.
CCB028RaCLIA Kit for Albumin (ALB)Chemiluminescent immunoassay for Antigen Detection.
KSB028Ra11ELISA Kit DIY Materials for Albumin (ALB)Main materials for "Do It (ELISA Kit) Yourself".
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