ELISA Kit for Peroxiredoxin 5 (PRDX5)
PLP; PRDX6; ACR1; PMP20; PRXV; SBBI10; Thioredoxin Peroxidase PMP20; Peroxisomal Antioxidant Enzyme; TPx Type VI; Liver Tissue 2D-Page Spot 71B; Alu Co-Repressor 1
- Product No.SEF755Ra
- Organism SpeciesRattus norvegicus (Rat) Same name, Different species.
- Test MethodDouble-antibody Sandwich
- Assay Length3h
- Detection Range78-5,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 34pg/mL.
- Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
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FOB
US$ 532
For more details, please contact local distributors!US$ 760
For more details, please contact local distributors! US$ 3420
For more details, please contact local distributors! US$ 6460
For more details, please contact local distributors! US$ 53200
For more details, please contact local distributors!
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Packages (Simulation)
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Packages (Simulation)
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Results demonstration
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Typical Standard Curve
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ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
This assay has high sensitivity and excellent specificity for detection of Peroxiredoxin 5 (PRDX5).
No significant cross-reactivity or interference between Peroxiredoxin 5 (PRDX5) and analogues was observed.
Recovery of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
Matrices listed below were spiked with certain level of recombinant Peroxiredoxin 5 (PRDX5) and the recovery rates were calculated by comparing the measured value to the expected amount of Peroxiredoxin 5 (PRDX5) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 92-105 | 101 |
| EDTA plasma(n=5) | 84-91 | 88 |
| heparin plasma(n=5) | 78-104 | 98 |
Precision of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Peroxiredoxin 5 (PRDX5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Peroxiredoxin 5 (PRDX5) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 91-102% | 98-105% | 87-94% | 96-104% |
| EDTA plasma(n=5) | 90-97% | 80-98% | 89-97% | 84-98% |
| heparin plasma(n=5) | 97-105% | 96-105% | 87-101% | 84-99% |
Stability of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle of the ELISA Kit for Peroxiredoxin 5 (PRDX5)
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5 (PRDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 5 (PRDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 5 (PRDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 5 (PRDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Related products
| Catalog No. | Organism species: Rattus norvegicus (Rat) | Applications (RESEARCH USE ONLY!) |
| RPF755Ra01 | Recombinant Peroxiredoxin 5 (PRDX5) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF755Ra01 | Polyclonal Antibody to Peroxiredoxin 5 (PRDX5) | WB; IHC; ICC; IP. |
| LAF755Ra71 | Biotin-Linked Polyclonal Antibody to Peroxiredoxin 5 (PRDX5) | WB; IHC; ICC. |
| SEF755Ra | ELISA Kit for Peroxiredoxin 5 (PRDX5) | Enzyme-linked immunosorbent assay for Antigen Detection. |
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| KSF755Ra01 | ELISA Kit DIY Materials for Peroxiredoxin 5 (PRDX5) | Main materials for "Do It (ELISA Kit) Yourself". |