ELISA Kit for Stem Cell Factor (SCF)

KITLG; MGF; SF; KL1; Kitl; FPH2; Kit-Ligand; Steel Factor; Mast Cell Growth Factor; Steel factor; Familial Progressive Hyperpigmentation 2

  • ELISA Kit for Stem Cell Factor (SCF) Packages (Simulation)
  • ELISA Kit for Stem Cell Factor (SCF) Packages (Simulation)
  • ELISA Kit for Stem Cell Factor (SCF) Results demonstration
  • SEA120Mu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Stem Cell Factor (SCF)

This assay has high sensitivity and excellent specificity for detection of Stem Cell Factor (SCF).
No significant cross-reactivity or interference between Stem Cell Factor (SCF) and analogues was observed.

Recovery of the ELISA Kit for Stem Cell Factor (SCF)

Matrices listed below were spiked with certain level of recombinant Stem Cell Factor (SCF) and the recovery rates were calculated by comparing the measured value to the expected amount of Stem Cell Factor (SCF) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 94-104 101
EDTA plasma(n=5) 89-103 99
heparin plasma(n=5) 85-102 93

Precision of the ELISA Kit for Stem Cell Factor (SCF)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Stem Cell Factor (SCF)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Stem Cell Factor (SCF) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 87-102% 96-105% 93-101% 90-97%
EDTA plasma(n=5) 84-98% 85-99% 94-102% 90-97%
heparin plasma(n=5) 79-101% 89-97% 84-96% 87-101%

Stability of the ELISA Kit for Stem Cell Factor (SCF)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Stem Cell Factor (SCF)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Stem Cell Factor (SCF)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stem Cell Factor (SCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stem Cell Factor (SCF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Mus musculus (Mouse) Applications (RESEARCH USE ONLY!)
RPA120Mu02 Recombinant Stem Cell Factor (SCF) Positive Control; Immunogen; SDS-PAGE; WB.
RPA120Mu01 Recombinant Stem Cell Factor (SCF) Positive Control; Immunogen; SDS-PAGE; WB.
APA120Mu01 Active Stem Cell Factor (SCF) Cell culture; Activity Assays.
PAA120Mu02 Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IP.
PAA120Mu01 Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IP.
LAA120Mu81 FITC-Linked Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC; IF.
LAA120Mu71 Biotin-Linked Polyclonal Antibody to Stem Cell Factor (SCF) WB; IHC; ICC.
MEA120Mu Mini Samples ELISA Kit for Stem Cell Factor (SCF) Enzyme-linked immunosorbent assay for Antigen Detection.
SEA120Mu ELISA Kit for Stem Cell Factor (SCF) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA120Mu CLIA Kit for Stem Cell Factor (SCF) Chemiluminescent immunoassay for Antigen Detection.
LMA120Mu Multiplex Assay Kit for Stem Cell Factor (SCF) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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