ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

Intermediate Filament Protein

  • ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)Packages (Simulation)
  • ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)Packages (Simulation)
  • ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)Results demonstration
  • SEA068Mu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

This assay has high sensitivity and excellent specificity for detection of Glial Fibrillary Acidic Protein (GFAP).
No significant cross-reactivity or interference between Glial Fibrillary Acidic Protein (GFAP) and analogues was observed.

Recovery of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

Matrices listed below were spiked with certain level of recombinantGlial Fibrillary Acidic Protein (GFAP) and the recovery rates were calculated by comparing the measured value to the expected amount of Glial Fibrillary Acidic Protein (GFAP) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-10283
EDTA plasma(n=5)87-9692
heparin plasma(n=5)85-9888

Precision of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glial Fibrillary Acidic Protein (GFAP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glial Fibrillary Acidic Protein (GFAP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glial Fibrillary Acidic Protein (GFAP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)88-101%91-98%78-101%78-92%
EDTA plasma(n=5)88-101%94-102%78-94%92-105%
heparin plasma(n=5)99-105%88-105%96-105%96-104%

Stability of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Glial Fibrillary Acidic Protein (GFAP)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glial Fibrillary Acidic Protein (GFAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glial Fibrillary Acidic Protein (GFAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glial Fibrillary Acidic Protein (GFAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glial Fibrillary Acidic Protein (GFAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No.Organism species: Mus musculus (Mouse)Applications (RESEARCH USE ONLY!)
RPA068Mu03Recombinant Glial Fibrillary Acidic Protein (GFAP)Positive Control; Immunogen; SDS-PAGE; WB.
RPA068Mu02Recombinant Glial Fibrillary Acidic Protein (GFAP)Positive Control; Immunogen; SDS-PAGE; WB.
RPA068Mu01Recombinant Glial Fibrillary Acidic Protein (GFAP)Positive Control; Immunogen; SDS-PAGE; WB.
RPA068Mu04Recombinant Glial Fibrillary Acidic Protein (GFAP)Positive Control; Immunogen; SDS-PAGE; WB.
PAA068Mu03Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)WB; IHC; ICC; IP.
PAA068Mu02Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)WB; IHC; ICC; IP.
PAA068Mu01Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)WB; IHC; ICC; IP.
PAA068Mu04Polyclonal Antibody to Glial Fibrillary Acidic Protein (GFAP)WB; IHC; ICC; IP.
SEA068MuELISA Kit for Glial Fibrillary Acidic Protein (GFAP)Enzyme-linked immunosorbent assay for Antigen Detection.
KSA068Mu01ELISA Kit DIY Materials for Glial Fibrillary Acidic Protein (GFAP)Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK