ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

MAPK1; P38; P40; ERK2; ERT1; MAPK2; P42MAPK; P42-MAPK; PRKM1; PRKM2; P41; P41mapk; P41-mapk; Mitogen-Activated Protein Kinase 1; Mitogen-activated protein kinase 2

  • ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)Packages (Simulation)
  • ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)Packages (Simulation)
  • ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)Results demonstration
  • SEA930Hu.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

This assay has high sensitivity and excellent specificity for detection of Extracellular Signal Regulated Kinase 2 (ERK2).
No significant cross-reactivity or interference between Extracellular Signal Regulated Kinase 2 (ERK2) and analogues was observed.

Recovery of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

Matrices listed below were spiked with certain level of recombinantExtracellular Signal Regulated Kinase 2 (ERK2) and the recovery rates were calculated by comparing the measured value to the expected amount of Extracellular Signal Regulated Kinase 2 (ERK2) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)94-10299
EDTA plasma(n=5)86-9389
heparin plasma(n=5)87-9692

Precision of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 2 (ERK2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Extracellular Signal Regulated Kinase 2 (ERK2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Extracellular Signal Regulated Kinase 2 (ERK2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)85-95%79-95%87-94%99-105%
EDTA plasma(n=5)80-105%83-93%78-96%78-92%
heparin plasma(n=5)99-105%80-97%88-95%90-104%

Stability of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

Test principle of the ELISA Kit for Extracellular Signal Regulated Kinase 2 (ERK2)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Extracellular Signal Regulated Kinase 2 (ERK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Extracellular Signal Regulated Kinase 2 (ERK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Extracellular Signal Regulated Kinase 2 (ERK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Extracellular Signal Regulated Kinase 2 (ERK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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