ELISA Kit for Clara Cell Protein 16 (CC16)

SCGB1A1; CC10; CCSP; UGB; UG; Uteroglobin; Uteroglobin; Urinary Protein 1; Clara Cell Secretory Protein; Secretoglobin Family 1A Member 1; Clara-Cell Specific 10-kD Protein

  • ELISA Kit for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • ELISA Kit for Clara Cell Protein 16 (CC16) Packages (Simulation)
  • ELISA Kit for Clara Cell Protein 16 (CC16) Results demonstration
  • SEA857Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the ELISA Kit for Clara Cell Protein 16 (CC16)

This assay has high sensitivity and excellent specificity for detection of Clara Cell Protein 16 (CC16).
No significant cross-reactivity or interference between Clara Cell Protein 16 (CC16) and analogues was observed.

Recovery of the ELISA Kit for Clara Cell Protein 16 (CC16)

Matrices listed below were spiked with certain level of recombinant Clara Cell Protein 16 (CC16) and the recovery rates were calculated by comparing the measured value to the expected amount of Clara Cell Protein 16 (CC16) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 80-88 84
EDTA plasma(n=5) 84-101 91
heparin plasma(n=5) 94-105 98

Precision of the ELISA Kit for Clara Cell Protein 16 (CC16)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Clara Cell Protein 16 (CC16) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the ELISA Kit for Clara Cell Protein 16 (CC16)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Clara Cell Protein 16 (CC16) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 83-97% 78-95% 92-101% 92-99%
EDTA plasma(n=5) 81-97% 78-98% 95-103% 79-103%
heparin plasma(n=5) 83-101% 88-102% 84-98% 78-97%

Stability of the ELISA Kit for Clara Cell Protein 16 (CC16)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the ELISA Kit for Clara Cell Protein 16 (CC16)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Test principle of the ELISA Kit for Clara Cell Protein 16 (CC16)

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Clara Cell Protein 16 (CC16). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Clara Cell Protein 16 (CC16). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Clara Cell Protein 16 (CC16), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Clara Cell Protein 16 (CC16) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPA857Hu01 Recombinant Clara Cell Protein 16 (CC16) Positive Control; Immunogen; SDS-PAGE; WB.
PAA857Hu01 Polyclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC; ICC; IP.
LAA857Hu81 FITC-Linked Polyclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC; ICC; IF.
LAA857Hu71 Biotin-Linked Polyclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC; ICC.
MAA857Hu21 Monoclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC
MAA857Hu23 Monoclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC; ICC; IP.
MAA857Hu22 Monoclonal Antibody to Clara Cell Protein 16 (CC16) WB; IHC; ICC; IP.
SEA857Hu ELISA Kit for Clara Cell Protein 16 (CC16) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA857Hu Multiplex Assay Kit for Clara Cell Protein 16 (CC16) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
KSA857Hu01 ELISA Kit DIY Materials for Clara Cell Protein 16 (CC16) Main materials for "Do It (ELISA Kit) Yourself".
GUESTBOOK