ELISA Kit for Growth Regulated Oncogene Gamma (GROg)

CXCL3; SCYB3; GRO3; GRO-G; MIP2-B; MIP2b; CINC2b; CINC2b; Chemokine(C-X-C-Motif)ligand 3; Macrophage inflammatory protein 2-beta

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
  • ELISA Kit for Growth Regulated Oncogene Gamma (GROg)Packages (Simulation)
  • ELISA Kit for Growth Regulated Oncogene Gamma (GROg)Packages (Simulation)
  • ELISA Kit for Growth Regulated Oncogene Gamma (GROg)Results demonstration
  • SEB604Ra.jpgTypical Standard Curve
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity

This assay has high sensitivity and excellent specificity for detection of Growth Regulated Oncogene Gamma (GROg).
No significant cross-reactivity or interference between Growth Regulated Oncogene Gamma (GROg) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Growth Regulated Oncogene Gamma (GROg) and the recovery rates were calculated by comparing the measured value to the expected amount of Growth Regulated Oncogene Gamma (GROg) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)84-10291
EDTA plasma(n=5)91-10196
heparin plasma(n=5)92-10197

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Growth Regulated Oncogene Gamma (GROg) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Growth Regulated Oncogene Gamma (GROg) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Growth Regulated Oncogene Gamma (GROg) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)93-102%79-95%88-99%85-96%
EDTA plasma(n=5)91-104%78-91%82-97%90-98%
heparin plasma(n=5)80-92%97-104%88-99%86-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

ReagentsQuantityReagentsQuantity
Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
Standard2Standard Diluent1×20mL
Detection Reagent A1×120µLAssay Diluent A1×12mL
Detection Reagent B1×120µLAssay Diluent B1×12mL
TMB Substrate1×9mLStop Solution1×6mL
Wash Buffer (30 × concentrate)1×20mLInstruction manual1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

ELISA Kit for Growth Regulated Oncogene Gamma (GROg)

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Regulated Oncogene Gamma (GROg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Regulated Oncogene Gamma (GROg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Regulated Oncogene Gamma (GROg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Regulated Oncogene Gamma (GROg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Related products

Catalog No.Organism species: Rattus norvegicus (Rat)Applications
RPB604Ra01Recombinant Growth Regulated Oncogene Gamma (GROg)Positive Control; Immunogen; SDS-PAGE; WB.
PAB604Ra01Polyclonal Antibody to Growth Regulated Oncogene Gamma (GROg)WB; IHC; ICC; IP.
MAB604Ra21Monoclonal Antibody to Growth Regulated Oncogene Gamma (GROg)WB; IHC; ICC; IP.
SEB604RaELISA Kit for Growth Regulated Oncogene Gamma (GROg)Enzyme-linked immunosorbent assay for Antigen Detection.
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