ELISA Kit DIY Materials for Protein Kinase C Alpha (PKCa)
PRKCA; PKC-A; AAG6; PKC-Alpha
- Product No.KSB918Mu01
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Reagent Contents Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
- Detectable SampleTissue homogenates and other biological fluids.
- Applicable PrincipleDouble-antibody Sandwich ELISA for Antigen Detection
- Detectable Range0.781-50ng/mL
- Applicable Sensitivity0.31ng/mL
- ApplicationsMain materials for "Do It(ELISA Kit) Yourself"
- Download n/a
- UOM 96T*596T*10 96T*20 96T*50 96T*100
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Packages (Simulation)
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Packages (Simulation)
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ISO9001: 2008, ISO13485: 2003 Registered
SPECIFITY of the ELISA Kit DIY Materials for Protein Kinase C Alpha (PKCa)
The Abs in the kit have high sensitivity and excellent specificity for detection of Protein Kinase C Alpha (PKCa). No significant cross-reactivity or interference between Protein Kinase C Alpha (PKCa) and analogues was observed.
USAGE of the ELISA Kit DIY Materials for Protein Kinase C Alpha (PKCa)
1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
STORAGE of the ELISA Kit DIY Materials for Protein Kinase C Alpha (PKCa)
Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.
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