ELISA Kit DIY Materials for Osteopontin (OPN)

SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin

  • ELISA Kit DIY Materials for Osteopontin (OPN) Packages (Simulation)
  • ELISA Kit DIY Materials for Osteopontin (OPN) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

SPECIFITY of the ELISA Kit DIY Materials for Osteopontin (OPN)

The Abs in the kit have high sensitivity and excellent specificity for detection of Osteopontin (OPN). No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.

USAGE of the ELISA Kit DIY Materials for Osteopontin (OPN)

1. Coat the plates with 100μL per well of working solution of Capture Antibody.incubate overnight at 4°C or incubate at 37°C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 μL per well of working solution of Blocking Buffer. Incubate at 37°C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.

STORAGE of the ELISA Kit DIY Materials for Osteopontin (OPN)

Antibodies, Standard and Streptavidin-HRP should be stored at -20°C. TMB should be stored at 4°C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4°C.

SUPPORT PACK of the ELISA Kit DIY Materials for Osteopontin (OPN)

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