CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

Serine/threonine-protein kinase PAK 4

  • CLIA Kit for p21 Protein Activated Kinase 4 (PAK4) Packages (Simulation)
  • CLIA Kit for p21 Protein Activated Kinase 4 (PAK4) Packages (Simulation)
  • CLIA Kit for p21 Protein Activated Kinase 4 (PAK4) Results demonstration
  • SCH464Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

This assay has high sensitivity and excellent specificity for detection of p21 Protein Activated Kinase 4 (PAK4).
No significant cross-reactivity or interference between p21 Protein Activated Kinase 4 (PAK4) and analogues was observed.

Recovery of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

Matrices listed below were spiked with certain level of recombinant p21 Protein Activated Kinase 4 (PAK4) and the recovery rates were calculated by comparing the measured value to the expected amount of p21 Protein Activated Kinase 4 (PAK4) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 98-105 101
EDTA plasma(n=5) 80-91 85
heparin plasma(n=5) 78-104 98

Precision of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level p21 Protein Activated Kinase 4 (PAK4) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level p21 Protein Activated Kinase 4 (PAK4) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of p21 Protein Activated Kinase 4 (PAK4) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 86-101% 79-99% 84-95% 91-98%
EDTA plasma(n=5) 91-102% 80-101% 97-104% 96-105%
heparin plasma(n=5) 97-105% 79-98% 92-105% 78-91%

Stability of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for p21 Protein Activated Kinase 4 (PAK4)

The microplate provided in this kit has been pre-coated with an antibody specific to p21 Protein Activated Kinase 4 (PAK4). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to p21 Protein Activated Kinase 4 (PAK4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the p21 Protein Activated Kinase 4 (PAK4) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
RPH464Hu01 Recombinant p21 Protein Activated Kinase 4 (PAK4) Positive Control; Immunogen; SDS-PAGE; WB.
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