CLIA Kit for Sulfatase 2 (SULF2)

HSULF-2; Extracellular sulfatase Sulf-2

  • CLIA Kit for Sulfatase 2 (SULF2)Packages (Simulation)
  • CLIA Kit for Sulfatase 2 (SULF2)Packages (Simulation)
  • CLIA Kit for Sulfatase 2 (SULF2)Results demonstration
  • CertificateISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Sulfatase 2 (SULF2)

This assay has high sensitivity and excellent specificity for detection of Sulfatase 2 (SULF2).
No significant cross-reactivity or interference between Sulfatase 2 (SULF2) and analogues was observed.

Recovery of the CLIA Kit for Sulfatase 2 (SULF2)

Matrices listed below were spiked with certain level of recombinantSulfatase 2 (SULF2) and the recovery rates were calculated by comparing the measured value to the expected amount of Sulfatase 2 (SULF2) in samples.

MatrixRecovery range (%)Average(%)
serum(n=5)80-10487
EDTA plasma(n=5)91-10496
heparin plasma(n=5)78-8982

Precision of the CLIA Kit for Sulfatase 2 (SULF2)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sulfatase 2 (SULF2) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Sulfatase 2 (SULF2)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Sulfatase 2 (SULF2) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample1:21:41:81:16
serum(n=5)96-105%84-102%83-101%98-105%
EDTA plasma(n=5)98-105%96-104%84-91%97-105%
heparin plasma(n=5)83-99%83-97%89-97%83-101%

Stability of the CLIA Kit for Sulfatase 2 (SULF2)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Sulfatase 2 (SULF2)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Sulfatase 2 (SULF2)

The microplate provided in this kit has been pre-coated with an antibody specific to Sulfatase 2 (SULF2). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Sulfatase 2 (SULF2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Sulfatase 2 (SULF2) level in the sample or standard.;

Related products

Catalog No.Organism species: Homo sapiens (Human)Applications (RESEARCH USE ONLY!)
RPH107Hu01Recombinant Sulfatase 2 (SULF2)Positive Control; Immunogen; SDS-PAGE; WB.
PAH107Hu01Polyclonal Antibody to Sulfatase 2 (SULF2)WB; IHC; ICC; IP.
LAH107Hu81FITC-Linked Polyclonal Antibody to Sulfatase 2 (SULF2)WB; IHC; ICC; IF.
LAH107Hu71Biotin-Linked Polyclonal Antibody to Sulfatase 2 (SULF2)WB; IHC; ICC.
MAH107Hu21Monoclonal Antibody to Sulfatase 2 (SULF2)WB; IHC; ICC; IP.
SEH107HuELISA Kit for Sulfatase 2 (SULF2)Enzyme-linked immunosorbent assay for Antigen Detection.
SCH107HuCLIA Kit for Sulfatase 2 (SULF2)Chemiluminescent immunoassay for Antigen Detection.
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