CLIA Kit for Interleukin 28B (IL28B)

IFNL3; Interferon,Lambda 3; Cytokine Zcyto22

  • CLIA Kit for Interleukin 28B (IL28B) Packages (Simulation)
  • CLIA Kit for Interleukin 28B (IL28B) Packages (Simulation)
  • CLIA Kit for Interleukin 28B (IL28B) Results demonstration
  • SCC028Hu.jpg Typical Standard Curve
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specificity of the CLIA Kit for Interleukin 28B (IL28B)

This assay has high sensitivity and excellent specificity for detection of Interleukin 28B (IL28B).
No significant cross-reactivity or interference between Interleukin 28B (IL28B) and analogues was observed.

Recovery of the CLIA Kit for Interleukin 28B (IL28B)

Matrices listed below were spiked with certain level of recombinant Interleukin 28B (IL28B) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 28B (IL28B) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 78-104 91
EDTA plasma(n=5) 84-102 98
heparin plasma(n=5) 92-105 101

Precision of the CLIA Kit for Interleukin 28B (IL28B)

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 28B (IL28B) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 28B (IL28B) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity of the CLIA Kit for Interleukin 28B (IL28B)

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 28B (IL28B) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 97-104% 78-99% 84-92%
EDTA plasma(n=5) 98-105% 94-105% 96-104% 84-98%
heparin plasma(n=5) 80-95% 78-91% 79-95% 97-105%

Stability of the CLIA Kit for Interleukin 28B (IL28B)

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay procedure summary of the CLIA Kit for Interleukin 28B (IL28B)

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Test principle of the CLIA Kit for Interleukin 28B (IL28B)

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 28B (IL28B). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 28B (IL28B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 28B (IL28B) level in the sample or standard.;

Related products

Catalog No. Organism species: Homo sapiens (Human) Applications (RESEARCH USE ONLY!)
APC028Hu01 Active Interleukin 28B (IL28B) Cell culture; Activity Assays.
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HEC028Hu High Sensitive ELISA Kit for Interleukin 28B (IL28B) Enzyme-linked immunosorbent assay for Antigen Detection.
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SCC028Hu CLIA Kit for Interleukin 28B (IL28B) Chemiluminescent immunoassay for Antigen Detection.
LMC028Hu Multiplex Assay Kit for Interleukin 28B (IL28B) ,etc. by FLIA (Flow Luminescence Immunoassay) FLIA Kit for Antigen Detection.
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