CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

Glycosylated Hemoglobin; Hemoglobin A1c; Hb1c; HbAIc; HbAIc

FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
    • CLIA Kit for Glycated Hemoglobin A1c (HbA1c)Packages (Simulation)
    • CLIA Kit for Glycated Hemoglobin A1c (HbA1c)Packages (Simulation)
    • CLIA Kit for Glycated Hemoglobin A1c (HbA1c)Results demonstration
    • CCA190Hu.jpgTypical Standard Curve
    • CertificateISO9001: 2008, ISO13485: 2003 Registered

    Specificity

    This assay has high sensitivity and excellent specificity for detection of Glycated Hemoglobin A1c (HbA1c).
    No significant cross-reactivity or interference between Glycated Hemoglobin A1c (HbA1c) and analogues was observed.

    Recovery

    Matrices listed below were spiked with certain level of recombinant Glycated Hemoglobin A1c (HbA1c) and the recovery rates were calculated by comparing the measured value to the expected amount of Glycated Hemoglobin A1c (HbA1c) in samples.

    MatrixRecovery range (%)Average(%)
    serum(n=5)78-9381
    EDTA plasma(n=5)83-9388
    heparin plasma(n=5)83-9188

    Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glycated Hemoglobin A1c (HbA1c) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Linearity

    The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Glycated Hemoglobin A1c (HbA1c) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

    Sample1:21:41:81:16
    serum(n=5)78-91%85-93%88-102%81-103%
    EDTA plasma(n=5)82-94%78-101%84-97%79-99%
    heparin plasma(n=5)80-90%82-93%91-105%78-96%

    Stability

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Reagents and materials provided

    ReagentsQuantityReagentsQuantity
    Pre-coated, ready to use 96-well strip plate1Plate sealer for 96 wells4
    Standard2Standard Diluent1×20mL
    Detection Reagent A1×120µLAssay Diluent A1×12mL
    Detection Reagent B1×120µLAssay Diluent B1×12mL
    Substrate A1×10mLSubstrate B1×2mL
    Wash Buffer (30 × concentrate)1×20mLInstruction manual1

    Assay procedure summary

    1. Prepare all reagents, samples and standards;
    2. Add 50µL standard or sample to each well.
        And then add 50µL prepared Detection Reagent A immediately.
        Shake and mix. Incubate 1 hour at 37°C;
    3. Aspirate and wash 3 times;
    4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
    5. Aspirate and wash 5 times;
    6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
    7. Read RLU value immediately.

    CLIA Kit for Glycated Hemoglobin A1c (HbA1c)

    Test principle

    The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Glycated Hemoglobin A1c (HbA1c). A competitive inhibition reaction is launched between biotin labeled Glycated Hemoglobin A1c (HbA1c) and unlabeled Glycated Hemoglobin A1c (HbA1c) (Standards or samples) with the pre-coated antibody specific to Glycated Hemoglobin A1c (HbA1c). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Glycated Hemoglobin A1c (HbA1c) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Glycated Hemoglobin A1c (HbA1c) level in the sample or standard.

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