CLIA Kit for Follicle Stimulating Hormone (FSH)
- Product No.CCA830Mu
- Organism SpeciesMus musculus (Mouse) Same name, Different species.
- Test MethodCompetitive Inhibition
- Assay Length2h
- Detection Range703.1-180,000pg/mL
- SensitivityThe minimum detectable dose of this kit is typically less than 284.3pg/mL.
- Sample TypeSerum, plasma and other biological fluids
- Download Instruction Manual
- UOM 48T96T 96T*5 96T*10 96T*100
-
FOB
US$ 545
For more details, please contact local distributors!US$ 778
For more details, please contact local distributors! US$ 3501
For more details, please contact local distributors! US$ 6613
For more details, please contact local distributors! US$ 54460
For more details, please contact local distributors!
-
Packages (Simulation)
-
Packages (Simulation)
-
Results demonstration
-
Typical Standard Curve
-
ISO9001: 2008, ISO13485: 2003 Registered
Specificity of the CLIA Kit for Follicle Stimulating Hormone (FSH)
This assay has high sensitivity and excellent specificity for detection of Follicle Stimulating Hormone (FSH).
No significant cross-reactivity or interference between Follicle Stimulating Hormone (FSH) and analogues was observed.
Recovery of the CLIA Kit for Follicle Stimulating Hormone (FSH)
Matrices listed below were spiked with certain level of recombinant Follicle Stimulating Hormone (FSH) and the recovery rates were calculated by comparing the measured value to the expected amount of Follicle Stimulating Hormone (FSH) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-94 | 91 |
| EDTA plasma(n=5) | 85-101 | 91 |
| heparin plasma(n=5) | 93-101 | 97 |
Precision of the CLIA Kit for Follicle Stimulating Hormone (FSH)
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Follicle Stimulating Hormone (FSH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity of the CLIA Kit for Follicle Stimulating Hormone (FSH)
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Follicle Stimulating Hormone (FSH) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 82-96% | 92-101% | 85-101% |
| EDTA plasma(n=5) | 83-101% | 78-104% | 83-97% | 79-94% |
| heparin plasma(n=5) | 83-91% | 80-102% | 82-104% | 97-105% |
Stability of the CLIA Kit for Follicle Stimulating Hormone (FSH)
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary of the CLIA Kit for Follicle Stimulating Hormone (FSH)
1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
7. Read RLU value immediately.
Test principle of the CLIA Kit for Follicle Stimulating Hormone (FSH)
The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Follicle Stimulating Hormone (FSH). A competitive inhibition reaction is launched between biotin labeled Follicle Stimulating Hormone (FSH) and unlabeled Follicle Stimulating Hormone (FSH) (Standards or samples) with the pre-coated antibody specific to Follicle Stimulating Hormone (FSH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Follicle Stimulating Hormone (FSH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Follicle Stimulating Hormone (FSH) level in the sample or standard.
GIVEAWAYS
INCREMENT SERVICES
Related products
| Catalog No. | Organism species: Mus musculus (Mouse) | Applications (RESEARCH USE ONLY!) |
| RPA830Mu01 | Recombinant Follicle Stimulating Hormone (FSH) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA830Mu01 | Polyclonal Antibody to Follicle Stimulating Hormone (FSH) | WB; IHC; ICC; IP. |
| LAA830Mu71 | Biotin-Linked Polyclonal Antibody to Follicle Stimulating Hormone (FSH) | WB; IHC; ICC. |
| SEA830Mu | ELISA Kit for Follicle Stimulating Hormone (FSH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA830Mu | Mini Samples ELISA Kit for Follicle Stimulating Hormone (FSH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA830Mu | ELISA Kit for Follicle Stimulating Hormone (FSH) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA830Mu | CLIA Kit for Follicle Stimulating Hormone (FSH) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA830Mu | Multiplex Assay Kit for Follicle Stimulating Hormone (FSH) ,etc. by FLIA (Flow Luminescence Immunoassay) | FLIA Kit for Antigen Detection. |